BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
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Reversed-Phase HPLC<br />
Alberta Peptide Institute test mix<br />
<strong>Column</strong>: PLRP-S 100Å<br />
PL1512-5500<br />
4.6 x 250 mm, 5 µm<br />
Mobile Phase: A: 0.1% TFA in 99% water:1% ACN<br />
B: 0.1% TFA in 70% water:30% ACN<br />
Gradient: 0-100% B in 30 min<br />
Flow Rate: 1.0 mL/min<br />
Detector: UV, 220 nm<br />
Whey proteins in dairy samples – milk<br />
<strong>Column</strong>: PLRP-S 300Å<br />
PL1512-3801<br />
4.6 x 150 mm, 8 µm<br />
Mobile Phase: A: 0.1% TFA in 99% water:1% ACN<br />
B: 0.1% TFA in 1% water:99% ACN<br />
Gradient: 36-48% B, 0-24 min, 48-100% B, 24-30 min<br />
100% B, 30-35 min, 100-36% B, 35-40 min<br />
40 www.agilent.com/chem/biohplc<br />
0<br />
Flow Rate: 1.0 mL/min<br />
Injection Volume: 10 µL<br />
Detector: UV, 220 nm<br />
1 2<br />
min<br />
3<br />
4<br />
1<br />
5<br />
0 min 24<br />
2<br />
3<br />
1. Ala 3-Gly 4 (free amino)<br />
2. Gly 3-Gly 4 (Nα-acetylated)<br />
3. Ala 3-Gly 4 (Nα-acetylated)<br />
4. Val 3-Gly 4 (Nα-acetylated)<br />
5. Val 3-Val 4 (Nα-acetylated)<br />
30<br />
VLC0050<br />
1. α-Lactalbumin<br />
2. β-Lactoglobulin (B chain)<br />
3. β-Lactoglobulin (A chain)<br />
VLC0074
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