BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
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size exclusion Chromatography (seC)<br />
<strong>Guide</strong> to eluent selection for PL aquagel-OH applications<br />
Polymer Chemistry neutral<br />
anionic Cationic<br />
Suggested Eluent<br />
Hydrophobic Polymers<br />
Polyvinyl alcohol<br />
<strong>Column</strong>: 3 x PL aquagel-OH MIXED<br />
PL1149-6800<br />
7.5 x 300 mm, 8 µm<br />
Mobile Phase: 0.2 M NaNO 3, 0.01 M NaH 2PO 4, pH 7<br />
Flow Rate: 1.0 mL/min<br />
Detector: <strong>Agilent</strong> PL-GPC 50 (RI)<br />
4 www.agilent.com/chem/biohplc<br />
Pure Water<br />
10<br />
Water soluble polymer<br />
0. -0.3 M nanO 3<br />
pH -9<br />
0.0 M naH 2P0 4<br />
addition of up to 50% methanol<br />
Retention time/min<br />
0.2-0. M nanO 3<br />
pH 2-<br />
0.0 M naH 2P0 4<br />
33<br />
VLC0032
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