BioHPLC Column Selection Guide Cover - Agilent Technologies

Column Specifications
Agilent Bio SEC-3
size exclusion Chromatography (seC)
• Exceptional loading capacity, stability, and reproducibility for size-based biomolecule separations
• Sharper peaks, higher resolution, and better protein recovery
• Faster separations than large-particle SEC columns
• Compatibility with most aqueous buffers
• Excellent stability in high-salt and low-salt conditions
Agilent Bio SEC-3 HPLC columns are a breakthrough technology for size exclusion chromatography
(SEC). They are packed with spherical, narrowly dispersed 3 µm silica particles coated with a
proprietary hydrophilic layer. This thin polymeric layer is chemically bonded to pure, mechanically
stable silica under controlled conditions, ensuring a highly efficient size exclusion particle.
Agilent Bio SEC-3 HPLC columns are available in 100Å, 150Å and 300Å pore sizes to accommodate
most peptide and protein size exclusion separations.
Pore Size Particle Size MW Range pH Range Max Pressure Flow Rate
100Å 3 µm 100-100,000 2-8.5 240 bar, 3500 psi 0.1-1.25 mL/min (7.8 mm id)
0.1-0.4 mL/min (4.6 mm id)
150Å 3 µm 500-150,000 2-8.5 240 bar, 3500 psi 0.1-1.25 mL/min (7.8 mm id)
0.1-0.4 mL/min (4.6 mm id)
300Å 3 µm 5,000-1,250,000 2-8.5 240 bar, 3500 psi 0.1-1.25 mL/min (7.8 mm id)
0.1-0.4 mL/min (4.6 mm id)
Tips & Tools
Deactivated/silanized vials have inert surfaces that will not interact with metals, biologicals or proteins,
and will not cause pH shifts. avoid standard polypropylene vials for biological or light-sensitive compounds.
UHPLC/HPLC