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BioHPLC Column Selection Guide Cover - Agilent Technologies

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ZORBAX 300Å StableBond<br />

ZORBAX PrepHT<br />

• Easy scale-up from analytical to preparative scale with ZORBAX phases<br />

• Fast preparative separations, up to 2000 mg<br />

• 5 to 7 µm particles for high efficiency and high yield<br />

• Easy to install finger-tight connections seal up to 5000 psi/350 bar<br />

• Use to maintain selectivity of the analytical phase in your prep separations<br />

Hardware Description Size (mm) Particle Size (µm)<br />

PrepHT Cartridge <strong>Column</strong>s (require endfittings kit 820400-901)<br />

ZORBAX PrepHT<br />

High purity, high recovery, and high throughput can be easily achieved with <strong>Agilent</strong> ZORBAX PrepHT<br />

columns. These are available in a variety of bonded phases – StableBond 300Å, C18, C8, C3, and<br />

CN – for optimized resolution and loadability under any conditions.<br />

ZORBAX PrepHT columns are packed with 5 and 7 µm particle sizes for very high resolution. The high<br />

resolution allows high loadability, high yield, and high purity of compounds. The larger diameter<br />

columns and mechanically stronger ZORBAX particles allow for flow rates up to 100 mL/min, thus<br />

increasing throughput.<br />

ZORBAX PrepHT columns are designed for rapid scale-up from analytical to preparative scale without<br />

losing resolution. For complex separations on larger columns (21.2 mm id, 150 mm length and<br />

longer), <strong>Agilent</strong> has carefully chosen the 7 µm particle size to achieve a balance between high<br />

efficiency and high loadability.<br />

300SB-C18<br />

USP L1<br />

300SB-C8<br />

USP L7<br />

300SB-CN<br />

USP L10<br />

300SB-C3<br />

USP L56<br />

PrepHT Cartridge 21.2 x 250 7 897250-102 897250-106 897250-105 897250-109<br />

PrepHT Cartridge 21.2 x 150 7 897150-102 897150-106 897150-109<br />

PrepHT Cartridge 21.2 x 150 5 895150-902 895150-906 895150-909<br />

PrepHT Cartridge 21.2 x 100 5 895100-902 895100-906 895100-909<br />

PrepHT Cartridge 21.2 x 50 5 895050-902 895050-906 895050-909<br />

PrepHT endfittings, 2/pk 820400-901 820400-901 820400-901 820400-901<br />

PrepHT Guard Cartridge, 2/pk 17 x 7.5 5 820212-921 820212-918 820212-924 820212-924<br />

Guard Cartridge Hardware 820444-901 820444-901 820444-901 820444-901<br />

PURIFICATION<br />

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