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BioHPLC Column Selection Guide Cover - Agilent Technologies

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ZORBAX Bio-SCX Series II<br />

Capillary and Nano <strong>Column</strong>s<br />

ZORBAX has Bio-SCX Series II columns designed for optimized 2-D separations of peptides and<br />

proteins using LC/MS. This packing is based on ultra-pure 3.5 µm ZORBAX silica particles, bonded<br />

with a bio-friendly polymer that is functionalized with sulfonic acid groups. This gives strong retention<br />

and good peak shape in the ion-exchange step of 2-D analysis of peptides and proteins.<br />

<strong>Column</strong> Specifications<br />

Pore<br />

Max<br />

Bonded Phase<br />

Size Surface Area pH Range Functionality Pressure<br />

ZORBAX Bio-SCX Series II 300Å 90 m2/g 2.5-8.5 Sulfonic acid 350 bar<br />

ZORBAX Bio-SCX Series II<br />

Description Size (mm) Particle Size (µm)<br />

Bio-SCX<br />

Series II<br />

Capillary 0.3 x 35 3.5 5065-9912<br />

Capillary 0.8 x 50 3.5 5065-9942<br />

ANALYSIS SCALE<br />

113

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