BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
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Consistent ion-exchange MAb separation<br />
<strong>Column</strong>: Bio MAb, PEEK<br />
5190-2411<br />
2.1 x 250 mm, 5 µm<br />
Buffer: A: Sodium phosphate buffer, 20 mM<br />
B: Buffer A + 400 mM NaCl<br />
Gradient: 15-35% Buffer B from 0-30 min<br />
Flow Rate: 0.65 mL/min<br />
Sample: CHO-humanized MAb, 1 mg/mL<br />
Injection: 2.5 µL<br />
Detector: UV 220 nm<br />
Temperature: Ambient<br />
To provide a metal free flow path Bio MAb PEEK<br />
columns are available<br />
Virtually eliminate retention time variations<br />
<strong>Column</strong>: Bio MAb, stainless steel<br />
5190-2413<br />
4.6 x 250 mm, 10 µm<br />
Mobile Phase: A: 10 mM phosphate, pH 6.0<br />
B: A + 1.0 M NaCl<br />
Flow Rate: 1.0 mL/min<br />
Gradient: 0-100% B in 42 min<br />
Temperature: 25 °C<br />
Detector: UV 214 nm<br />
mAU<br />
120<br />
100<br />
80<br />
60<br />
40<br />
20<br />
0<br />
2<br />
mAU<br />
140<br />
120<br />
100<br />
80<br />
60<br />
40<br />
20<br />
0<br />
Lot A<br />
Lot B<br />
Lot C<br />
0 2<br />
2<br />
ion-exchange Chromatography<br />
1st Run<br />
4 6 8 10 12 14 16 18 min<br />
10th Run<br />
4 6 8 10 12 14 16 18 min<br />
1 2 3<br />
4 6 8 10 12 14 16 18 min<br />
1. Cytochrome c<br />
2. Lysozyme<br />
3. Ribonuclease A<br />
The combination of well-controlled resin production, column surface chemistry, and column packing virtually eliminates retention time variations<br />
from column-to-column and lot-to-lot.<br />
VLC_IE_Mab<br />
UHPLC/HPLC<br />
49
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