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BioHPLC Column Selection Guide Cover - Agilent Technologies

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ProSEC 300S<br />

• Mechanically robust polymer particles that do not bleed during use<br />

• Single column with extended linear resolving range<br />

• <strong>Column</strong> dimensions for use with multi-detector systems<br />

size exclusion Chromatography (seC)<br />

The <strong>Agilent</strong> ProSEC 300S column is specifically designed as a single column solution for globular<br />

protein analysis. The pore size selection and optimization provides an extended linear resolving range<br />

so that this single column can be used for analysis across the full range of globular proteins.<br />

The particles are extremely robust and do not fragment during use to leach particulates.<br />

This gives exceptionally stable baselines making this column an ideal choice for use with light<br />

scattering detectors.<br />

Two column dimensions, 7.5 mm id and 4.6 mm id, to suit multi-detector size exclusion<br />

chromatography provide an option for the analysis of small masses.<br />

ProSEC 300S <strong>Column</strong> Specifications<br />

Bonded Phase<br />

ProSEC 300S<br />

Pore<br />

Size<br />

Particle<br />

Size<br />

Protein MW<br />

Range<br />

pH<br />

Range Flow Rate<br />

ProSEC 300S 300Å 5 µm 1,500-800,000 2-7.5

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