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BioHPLC Column Selection Guide Cover - Agilent Technologies

BioHPLC Column Selection Guide Cover - Agilent Technologies

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Tips & Tools<br />

<strong>Agilent</strong> offers a variety of<br />

e-Seminars and on-site training<br />

to help you learn how to be a<br />

more effective chromatographer.<br />

For more information, visit<br />

www.agilent.com/chem/education<br />

Capillary and Nano <strong>Column</strong>s<br />

CAPILLARY AND NANO COLUMNS<br />

• Highest sensitivity for your smallest sample sizes<br />

• Compatible with all LC/MS interfaces<br />

• Internal diameters of 0.5, 0.3, 0.1, and 0.075 mm<br />

• Packings/phases for both small and large molecules (80Å and 300Å pore sizes, respectively)<br />

• Ideal for 1-D and 2-D (proteomics) applications<br />

<strong>Agilent</strong> ZORBAX Capillary (0.5 and 0.3 mm id) and Nano (0.1 and 0.075 mm id) columns are now<br />

available in a wide variety of phases, pore sizes, and dimensions. These columns are ideal for very<br />

sample-limited applications because they provide enhanced sensitivity by reducing on-column sample<br />

dilution. This high sensitivity can be provided with exceptional reproducibility using <strong>Agilent</strong> columns<br />

and low dispersion HPLC instruments. The fastest growing application for capillary and nano columns<br />

is 2-D LC/MS for complex proteomics samples. <strong>Agilent</strong> provides all the columns needed for the 2-D<br />

separation – the SCX columns for the first dimension, the reversed-phase trapping column, and the<br />

reversed-phase column for the second dimension.<br />

ANALYSIS SCALE<br />

107

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