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BioHPLC Column Selection Guide Cover - Agilent Technologies

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mRP-C18 High-Recovery Protein <strong>Column</strong>s<br />

affinity Chromatography<br />

The mRP (macroporous reversed-phase) C18 High-Recovery Protein column is designed for high<br />

recovery, high resolution separation, fractionation, and simultaneous desalting of complex protein<br />

samples (like immunodepleted serum or plasma proteins).<br />

• Greater than 95-99% protein sample recovery has been observed with immunodepleted<br />

serum using the <strong>Agilent</strong> Multiple Affinity Removal System - LC column<br />

• Can load up to 380 µg of total protein mass without reducing chromatographic resolution<br />

of the proteins<br />

• <strong>Column</strong> packed with macroporous C18-bonded ultrapure 5 µm particle silica designed<br />

to reduce or eliminate strong adsorption of proteins<br />

• Maximum operating pressure of 250 bar (4000 psi)<br />

• Compatible with water and all common organic solvents<br />

mRP-C18 High-Recovery Protein <strong>Column</strong>s<br />

Description Protein Load Capacity Part No.<br />

mRP-C18, 0.5 x 100 mm 10 ng - 5 µg 5188-6510<br />

mRP-C18, 2.1 x 75 mm 8 - 85 µg 5188-6511<br />

mRP-C18, 4.6 x 50 mm 40 - 380 µg 5188-5231<br />

UHPLC/HPLC<br />

9

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