BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
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Poroshell 300 columns separate<br />
proteins and peptides in seconds<br />
<strong>Column</strong>: Poroshell 300SB-C18<br />
660750-902<br />
2.1 x 75 mm, 5 µm<br />
Mobile Phase: A: 0.1% TFA in H20 B: 0.07% TFA in ACN<br />
Flow Rate: 3.0 mL/min<br />
Gradient: 5-100% B in 1.0 min<br />
Temperature: 70 °C, 260 bar pressure<br />
Detector: 215 nm<br />
Sample: Proteins and Peptides<br />
This separation of eight polypeptides and<br />
proteins is completed in less than 60<br />
seconds. Each peak is sharp and efficient.<br />
Tips & Tools<br />
1<br />
Reversed-Phase HPLC<br />
0 0.5<br />
Time (min)<br />
1.0<br />
Further information can be found in the following publications:<br />
Poroshell 300SB-C18 (59 -2 00enUs)<br />
Rapid HPLC Analysis of Monoclonal Antibody IgG1 Heavy Chains Using ZORBAX Poroshell 300SB-C8<br />
(59 9-00 0en)<br />
Use of Temperature to Increase Resolution in the Ultrafast HPLC Separation of Proteins with ZORBAX<br />
Poroshell 300SB-C8 HPLC <strong>Column</strong>s (59 9-05 9en)<br />
Using the High-pH Stability of ZORBAX Poroshell 300Extend-C18 to Increase Signal-to-Noise in LC/MS<br />
(59 9-0 3en)<br />
www.agilent.com/chem/library<br />
2<br />
3<br />
4<br />
5<br />
6<br />
7<br />
8<br />
1. Angiotensin II<br />
2. Neurotensin<br />
3. RNase<br />
4. Insulin<br />
5. Lysozyme<br />
6. Myoglobin<br />
7. Carbonic Anhydrase<br />
8. Ovalbumin<br />
LCPO001<br />
UHPLC/HPLC<br />
3
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