BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
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Heparin<br />
<strong>Column</strong>: 2 x PL aquagel-OH 30<br />
PL1120-6830<br />
7.5 x 300 mm, 8 µm<br />
Mobile Phase: 0.2 M NaNO 3, 0.01 M NaH 2PO 4, pH 7<br />
Flow Rate: 1.0 mL/min<br />
Detector: <strong>Agilent</strong> PL-GPC 50 (RI)<br />
Correlation of the SEC results<br />
with the poymer specification<br />
Sample Mn Mw<br />
A 22,500 28,200<br />
B 9,350 10,770<br />
Hyaluronic acid<br />
<strong>Column</strong>: PL aquagel-OH 60 15 µm<br />
PL1149-6260<br />
7.5 x 300 mm, 15 µm<br />
<strong>Column</strong>: PL aquagel-OH 40 15 µm<br />
PL1149-6240<br />
7.5 x 300 mm, 15 µm<br />
Mobile Phase: 0.2 M NaNO 3, 0.01 M NaH 2PO 4, pH 7<br />
Flow Rate: 1.0 mL/min<br />
Detector: <strong>Agilent</strong> PL-GPC 50 (RI)<br />
1<br />
dw/dlog M<br />
5<br />
A<br />
B<br />
0<br />
5<br />
Chromatogram<br />
size exclusion Chromatography (seC)<br />
Retention time/min<br />
5 min 18<br />
Mw 1,800,00 g/mol<br />
22<br />
VLC0033<br />
Log M 7<br />
VLC0034<br />
UHPLC/HPLC<br />
5
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