BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
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Broad Distribution Biomolecules<br />
Carbohydrates, Lipids and PEGs<br />
Broad Distribution Biomolecule <strong>Column</strong> <strong>Selection</strong><br />
Low MW polymers<br />
and oligomers,<br />
oligosaccharides,<br />
PEGs, lignosulfonates<br />
Biomolecule Separations<br />
Aqueous size exclusion chromatography employing columns packed with polymeric media can be<br />
extremely useful when investigating biomolecules and their derived species with broad molecular<br />
weight distributions. Examples include PEGylated proteins and complex polysaccharides which find<br />
use in biopharma applications. The wide pore size distribution of polymeric SEC columns compared<br />
to silica-based material are excellent for samples with polydispersities greater than one.<br />
Polydisperse biopolymers,<br />
polysaccharides, cellulose<br />
derivatives<br />
Very high MW polymers,<br />
hyaluronic acids,<br />
starches, gums<br />
2 or 3 PL aquagel-OH The PL aquagel-OH<br />
� PL aquagel-OH 8 µm<br />
� PL aquagel-OH 20 5 µm<br />
� PL aquagel-OH MIXED-M 8 µm<br />
2 or 3 PL aquagel-OH<br />
� PL aquagel-OH MIXED-H 8 µm<br />
� PL aquagel-OH 60/50/40 8 µm<br />
PL aquagel-OH 60/50/40 15 µm in series<br />
analytical series has a pH<br />
range of 2-10, compatible<br />
with organic solvents (up to<br />
50% methanol), mechanical<br />
stability up to 140 bar<br />
(2030 psi) and low column<br />
operating pressures.<br />
<strong>BioHPLC</strong> COLUMNS<br />
15
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