BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
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size exclusion Chromatography (seC)<br />
siZe eXCLUsiOn CHROMaTOGRaPHY (seC)<br />
Accurately determine biomolecule aggregation,<br />
fragmentation, and chemical ligation/modification<br />
Size exclusion chromatography (SEC) is a technique for separating proteins, oligonucleotides,<br />
and other complex biopolymers by size using aqueous eluents.<br />
Applying SEC to aggregation studies<br />
The size, type, and content of aggregates present in protein biopharmaceuticals can affect both<br />
efficacy and formulation – or worse, induce an immunogenic response. Aggregation formations occur<br />
through a variety of mechanisms, including disulfide bond formation and non-covalent interactions.<br />
Because the size of protein aggregates, including dimers, is sufficiently different from the protein<br />
monomer, you can separate the various forms using SEC. In fact, SEC with UV or light scattering<br />
is a standard technique for quantifying protein aggregation.<br />
Applying SEC to quantitation<br />
and molecular weight determination<br />
For proteins and other molecules of discreet molecular weight, SEC can be used to detect<br />
and quantitate monomers, dimers, aggregates and fragments. SEC can also separate<br />
oligonucleotide mixtures.<br />
For biopolymers of varying sizes, like starches and other polysaccharides, SEC can provide<br />
data on molecular weight distribution and branching (with the proper detectors).<br />
UHPLC/HPLC<br />
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