BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
You also want an ePaper? Increase the reach of your titles
YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.
size exclusion Chromatography (seC)<br />
Calibration curves – Bio SEC-3<br />
<strong>Column</strong>: Bio SEC-3<br />
7.8 x 300 mm, 3 µm<br />
Mobile Phase: 150 mM Na phosphate, pH 7.0<br />
Flow Rate: 1.0 mL/min<br />
Detector: UV<br />
Proteins MWt 300Å 150Å 100Å<br />
Thyroglobulin 670000 6.34 5.50 5.63<br />
Gamma globulin 158000 8.03 6.24 5.74<br />
BSA 67000 8.90 7.00 6.03<br />
Ovalbumin 45000 9.57 7.70 6.41<br />
Myoglobin 17000 10.12 8.50 7.10<br />
Ribonuclease A 12700 10.40 8.80 7.46<br />
Vitamin B-12 1350 11.90 11.40 10.20<br />
Intact MAb monomer and dimer separation<br />
<strong>Column</strong>: Bio SEC-3, 300Å<br />
5190-2511<br />
7.8 x 300 mm, 3 µm<br />
Buffer: Sodium phosphate buffer, pH 7.0, 150 mM<br />
Isocratic: 0-100% Buffer A from 0-30 min<br />
Flow Rate: 1.0 mL/min<br />
Sample: CHO-humanized MAb, 5 mg/mL – intact<br />
Injection: 5 µL<br />
Detector: UV 220 nm<br />
Temperature: Ambient<br />
www.agilent.com/chem/biohplc<br />
Molecular Weight<br />
1,000,000,000<br />
100,000,000<br />
10,000,000<br />
1,000,000<br />
100,000<br />
10,000<br />
1,000<br />
100<br />
4<br />
mAU<br />
300<br />
250<br />
200<br />
150<br />
100<br />
50<br />
300Å<br />
150Å<br />
100Å<br />
5 6 7 8 9 10 11 12 13<br />
MWvsRet<br />
Dimer<br />
Monomer<br />
Retention Volume (mL)<br />
0<br />
4 6 8 10 12 14 min<br />
VLC_MD_Mab
Change language