BioHPLC Column Selection Guide Cover - Agilent Technologies

BIOCOLUMN SELECTION GUIDELINES
From sample simplification to analysis, Agilent's biomolecule columns and supplies are easy to
integrate into your workflow for a complete, reproducible, and high-quality solution.
This comprehensive guide will help you find the right column for your biomolecule separation.
We have also included advice and tips on method development, solvent choice, mobile phase
modification, optimization, and many example separations, all to assist you in column selection
and method development.
Agilent has complete solutions for your needs. These include the Agilent 1260 Infinity Bio-inert LC
system with a metal-free sample path and the Agilent 1290 Infinity LC, designed to provide highest
speed, resolution, and ultra-sensitivity for UHPLC applications, including those utilizing Agilent
wide-pore 300Å ZORBAX StableBond columns. Biomolecules may be complex in structure, but
their analysis is simplified by using Agilent HPLC columns, systems, and supplies.
What is a biomolecule?
Biomolecules are compounds made by living organisms. They range in size from small lipids
to large polynucleotides such as DNA or RNA. They can be monomeric amino acids or
polymeric carbohydrates.
In this guide, we deal with the separation of:
Biocolumn Selection Guidelines
Proteins – separation based on size with size exclusion chromatography, charge with ion-exchange
chromatography, and hydrophobicity with reversed-phase chromatography.
Peptides – biocolumns for the analysis and purification of the full range of peptides, including
hydrophobic, hydrophilic, basic and acidic peptides across the full size range. Also, columns for
peptide mapping by HPLC and UHPLC.
DNA/RNA oligonucleotides – reversed-phase and ion-exchange options for DNA and RNA
oligos, and with particle pore sizes to cover the full range of oligonucleotide sizes, from small
synthetic oligos to large plasmids.
Amino acids – the ZORBAX Eclipse Amino Acid Analysis HPLC columns provide a high efficiency
solution for rapid analysis of 24 amino acids. Typical analysis times range from 14 minutes, with
a 75 mm column, to 24 minutes with a 150 mm column.
Broad-distribution polymers – analysis of lipids, polysaccharides and drug delivery compounds
using polymeric columns and standards to determine their molecular weight distribution and
composition. These compounds tend to exhibit broad MW distributions, in contrast to other
biomolecules that have narrow MW distributions or a defined molecular weight.
BioHPLC COLUMNS
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