BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
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Ion-Exchange Chromatography<br />
Charge isoform analysis<br />
of monoclonal antibodies<br />
<strong>Column</strong>: Bio MAb, PEEK<br />
5190-2407<br />
4.6 x 250 mm, 5 µm<br />
Mobile Phase: A: 10 mM Sodium Phosphate, pH 7.50<br />
B: A + 100 mM NaCl, pH 7.50<br />
Flow Rate: 0.8 mL/min<br />
Gradient: 15-95% B in 60 min<br />
Sample: 5 µL, 5 mg/mL, MAb<br />
50 www.agilent.com/chem/biohplc<br />
Absorbance (AU)<br />
0.60<br />
0.50<br />
0.40<br />
0.30<br />
0.20<br />
0.10<br />
0.00<br />
Acidic<br />
Isoforms<br />
Basic<br />
Isoforms<br />
0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34<br />
High resolution separation of acidic and basic charge variants using the <strong>Agilent</strong> Bio MAb NP5 column<br />
<strong>Agilent</strong> Bio MAb HPLC <strong>Column</strong>s<br />
Size (mm) Particle Size (µm)<br />
Bio MAb<br />
PEEK Pressure Limit<br />
Bio MAb<br />
Stainless Steel Pressure Limit<br />
4.6 x 250 10 5190-2415 275 bar, 4000 psi 5190-2413 275 bar, 4000 psi<br />
4.6 x 50, Guard 10 5190-2416 275 bar, 4000 psi<br />
4.6 x 250 5 5190-2407 413 bar, 6000 psi 5190-2405 413 bar, 6000 psi<br />
4.6 x 50, Guard 5 5190-2408 413 bar, 5800 psi<br />
4.6 x 50 3 5190-2403 551 bar, 8000 psi<br />
4.6 x 50 1.7 5190-2401 689 bar, 8700 psi<br />
4.0 x 10, Guard 10 5190-2414 275 bar, 4000 psi<br />
4.0 x 10, Guard 5 5190-2406 413 bar, 6000 psi<br />
4.0 x 10, Guard 3 5190-2404 551 bar, 8000 psi<br />
4.0 x 10, Guard 1.7 5190-2402 689 bar, 8700 psi<br />
2.1 x 250 10 5190-2419 275 bar, 4000 psi<br />
2.1 x 50, Guard 10 5190-2420 275 bar, 4000 psi<br />
2.1 x 250 5 5190-2411 413 bar, 5800 psi<br />
2.1 x 50, Guard 5 5190-2412 413 bar, 5800 psi<br />
Time (min)
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