BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
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High resolution of 24 amino acids<br />
using ZORBAX Eclipse-AAA protocol<br />
<strong>Column</strong>: ZORBAX Eclipse AAA<br />
963400-902<br />
4.6 x 150 mm, 3.5 µm<br />
Mobile Phase: A: 40 mM Na2HPO4, pH 7.8<br />
B: ACN:MeOH:Water,<br />
45:45:10 v/v<br />
Flow Rate: 2 mL/min<br />
Temperature: 40 °C<br />
Detector: Fluorescence<br />
Sample: 24 Amino Acids<br />
1<br />
0 5<br />
2<br />
3<br />
4<br />
5<br />
6<br />
7<br />
8<br />
9<br />
10<br />
11 12<br />
16 17<br />
18<br />
15<br />
19<br />
14<br />
20<br />
21<br />
10 15<br />
Time (min)<br />
22 23<br />
24<br />
Reversed-Phase HPLC<br />
1. Asp<br />
2. Glu<br />
3. Asn<br />
4. Ser<br />
5. Gln<br />
6. His<br />
7. Gly<br />
8. Thr<br />
9. Cit<br />
10. Arg<br />
11. Ala<br />
12. Tyr<br />
20 25<br />
This high resolution separation of 24 amino acids is done in 18 minutes. If the Rapid Resolution 4.6 x 75 mm Eclipse AAA column is selected,<br />
these amino acids are resolved in 9 minutes.<br />
Tips & Tools<br />
quick Reference <strong>Guide</strong>s list the common supplies you should have on hand to keep your agilent 200<br />
series LC operating at peak efficiency. Download your free copy at www.agilent.com/chem/getguides<br />
LCPAH01<br />
13. Cys<br />
14. Val<br />
15. Met<br />
16. Nva<br />
17. Trp<br />
18. Phe<br />
19. Ile<br />
20. Leu<br />
21. Lys<br />
22. Hyp<br />
23. Sar<br />
24. Pro<br />
UHPLC/HPLC<br />
45
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