BioHPLC Column Selection Guide Cover - Agilent Technologies

Poroshell 120
• 120Å pore size for shorter chain peptide mapping
• UHPLC performance on 600 bar systems
• Up to 90% of the efficiency of sub-2 µm
• 2X the efficiency of 3.5 µm
• Up to 50% less pressure than sub-2 µm columns
Reversed-Phase HPLC
Agilent Poroshell 120 columns are a 2.7 µm particle with a 1.7 µm solid core and 0.5 µm porous
outer layer. This small particle size provides high efficiency, similar to sub-2 µm columns, but with
40-50% less pressure. These high efficiency, high resolution columns can be used on any type of LC.
The porous outer layer and solid core limit diffusion distance and improve separation speed while
the narrow particle size distribution improves efficiency and resolution. The columns can support
high pressure and multiple columns can be used for the highest resolution and efficiency possible.
The smaller 120Å pore size is ideal for fast high resolution analysis of small hydrophilic peptides
in protein digests.
Column Specifications
Bonded Phase Pore Size Temp. Limits pH Range Endcapped
Carbon
Load
SB-C18 120Å 90 °C 1.0-8.0 No 8%
EC-C18 120Å 60 °C 2.0-8.0 Double 10%
Specifications represent typical values only.
UHPLC/HPLC
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