BioHPLC Column Selection Guide Cover - Agilent Technologies

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Appendices Cartridge/Guard Cartridge Systems Compatibility Guide* Icon Column Type Guard Cartridge Holder ID (mm) Phases 134 www.agilent.com/chem/biohplc Standard fitting Column guard cartridge (standalone) cartridge holder 820999-901 Semi-preparative column Semi-prep guard cartridge (standalone) cartridge holder 840140-901 PrepHT Guard cartridge 820444-901 Analytical Guard cartridge holder (PL1310-0016) and PLRP-S guard cartridges, 2/pk (PL1612-1801) Guard cartridge holder (PL1310-0016) and PL-SAX guard cartridges, 2/pk (PL1651-0102) Guard cartridge holder (PL1310-0016) and PL-SCX guard cartridges, 2/pk (PL1645-0102) *Standalone guard cartridges fit all cartridge and standard fitting columns available from Agilent. All columns without icons are standard fitting columns. 2.1 3.0 4.6 ZORBAX 9.4 ZORBAX 21.2 ZORBAX Agilent Prep 3.0 PLRP-S 3.0 PL-SAX 3.0 PL-SCX
USP DESIGNATIONS – BIOHPLC COLUMNS ONLY For a full listing of USP designations for all HPLC columns, please consult the Agilent catalog or www.agilent.com. The US Pharmacopeia (USP) is a standard source for many pharmaceutical methods that specifies columns by packing materials rather than by manufacturer. Listed below are the recommended Agilent Technologies columns suitable for most LC methods listed with the USP. Appendices USP Method USP Packing Materials Column Particle Size (µm) L1 Octadecyl silane chemically bonded to porous silica or ceramic micro-particles, Poroshell 120 EC-C18 2.7 1.5 to 10 µm in diameter Poroshell 120 SB-C18 2.7 ZORBAX 300SB-C18 1.8, 3.5, 5, 7 ZORBAX 300 Extend C18 3.5, 5 L7 Octyl silane chemically bonded to totally porous microsilica particles, Poroshell 120 EC-C8 2.7 1.5 to 10 µm in diameter ZORBAX 300SB C-8 1.8, 3.5, 5, 7 L10 Nitrile groups chemically bonded to porous silica particles, 3 to 10 µm in diameter ZORBAX 300 SB-CN 3.5, 5, 7 L14 Silica gel 10 µm in diameter with a chemically bonded, strongly basic quaternary ammonium anion-exchange coating ZORBAX SAX 5 L17 Strong cation-exchange resin consisting of sulfonated cross-linked Hi-Plex H 8 styrene-divinylbenzene copolymer in the hydrogen form, 7 to 11 µm in diameter Bio SCX NP10 10 L21 A rigid, spherical styrene-divinylbenzene copolymer, 5 to 10 µm in diameter PLRP-S 100Å 3, 5, 8, 10 PLRP-S 300Å 3, 5, 8, 10 PLRP-S 1000Å 5, 8, 10 PLRP-S 4000Å 5, 8, 10 L25 Packing having the capacity to separate compounds with a MW range from 1,000 to 5,000 da (as determined by the polyethylene oxide), applied to neutral, ionic and cationic water-soluble polymers PL aquagel-OH 5, 8 L33 Packing having the capacity to separate proteins by molecular size over a range ZORBAX GF-250 4 of 4,000 to 400,000 da. It is spherical, silica-based, and processed to provide pH stability Bio SEC-3 3 Bio SEC-5 5 ProSEC 300S 5 L35 A zirconium-stabilized spherical silica packing with a hydrophilic (diol-type) ZORBAX GF-250 4 molecular monolayer bonded phase ZORBAX GF-450 6 L50 A strong cation-exchange resin made of porous silica with sulfopropyl groups, 5 to 10 µm in diameter ZORBAX 300SCX 5 L56 Propyl silane chemically bonded to totally porous silica particles, 3 to 10 µm in diameter ZORBAX SB-C3 1.5, 3, 5 APPENDICES 135
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Agilent BioHPLC Column Selection Gu
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BIOCOLUMN SELECTION GUIDELINES From
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Column Selection Flow Chart Biocolu
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Protein Column Selection Guide Appl
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Separation of charge variants of hu
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Increased resolution for peptide ma
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Preparative scale purification of L
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DNA and RNA Oligonucleotide Column
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Broad Distribution Biomolecules Car
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ReveRseD-PHase HPLC Confidently per
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Sterically Protected 300StableBond
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Improved reproducibility of monoclo
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Short-chain ZORBAX 300SB-C3 is stab
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ZORBAX 300Å StableBond Hardware De
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LC/MS analysis of angiotensin on Ex
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Tips & Tools ZORBAX 300Å Extend-C1
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Poroshell 300 columns separate prot
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Monoclonal IgG1 chains: Separation
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Poroshell 120 • 120Å pore size f
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0 µm 0 µm 0 µm A B C Scanning el
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Exploiting chemical stability - TFA
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Temperature as a tool to enhance ma
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ZORBaX amino acid analysis (aaa) Co
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High resolution of 24 amino acids u
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Ion-Exchange Column Selection Appli
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Consistent ion-exchange MAb separat
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Column Specifications Tips & Tools
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Weak cation-exchange chromatography
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PL-SAX Strong Anion-Exchange Column
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Analysis of representative whey pro
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Standard protein separation Column:
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Agilent Bio-Monolith HPLC Column Se
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Baseline expansion of a separation
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size exclusion Chromatography (seC)
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Column Specifications Agilent Bio S
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Comparison of Agilent Bio SEC-3 and
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Column Length size exclusion Chroma
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Column Specifications Agilent Bio S
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Calibration curves - Bio SEC-5 Colu
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ProSEC 300S • Mechanically robust
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Overlay of UV and light scattering
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Retention volume versus log (MW) fo
Page 85 and 86: Agilent PL aquagel-OH SEC Columns f
Page 87 and 88: Heparin Column: 2 x PL aquagel-OH 3
Page 89 and 90: Polyethylene Glycol/Oxide standards
Page 91 and 92: aFFiniTY CHROMaTOGRaPHY Agilent Bio
Page 93 and 94: Rapid human polyclonal IgG quantifi
Page 95 and 96: Multiple Affinity Removal System af
Page 97 and 98: Illustration of high abundance prot
Page 99 and 100: mRP-C18 High-Recovery Protein Colum
Page 101 and 102: MeTHOD DeveLOPMenT ZORBAX Column Me
Page 103 and 104: Reversed-Phase LC/MS Methods Method
Page 105 and 106: Method Development Ionic Strength:
Page 107 and 108: Method Development After the initia
Page 109 and 110: Tips & Tools Agilent offers a varie
Page 111 and 112: Human serum: Low abundance protein
Page 113 and 114: 2-D LC/MS Analyses Using ZORBAX Cap
Page 115 and 116: ZORBAX Bio-SCX Series II Capillary
Page 117 and 118: ZORBAX HPLC Capillary Columns (glas
Page 119 and 120: Microbore HPLC for sensitive peptid
Page 121 and 122: BioPharmaceutical Lifecycle Reverse
Page 123 and 124: ZORBAX 300Å StableBond ZORBAX Prep
Page 125 and 126: PLRP-S Prep to Process Application
Page 127 and 128: Prep to Process PLRP-S Size (mm) Pa
Page 129 and 130: PL-SAX AND PL-SCX FOR PREP TO PROCE
Page 131 and 132: Prep to Process PL-SAX and PL-SCX D
Page 133 and 134: Crude peptide screen Column: VariTi
Page 135: CARTRIDGE COLUMN SYSTEMS Cartridge
Page 139 and 140: BioHPLC Columns Literature Title Ag
Page 141 and 142: BioHPLC Columns Literature Title An
Page 143 and 144: BioHPLC Columns Literature Title Co
Page 145 and 146: BIOPHARM DEFINITIONS A active start
Page 147 and 148: dimer A polymer made up of two iden
Page 149 and 150: K-L ligase An enzyme that causes mo
Page 151 and 152: S secondary structure In proteins,
Page 153 and 154: Poroshell 300 Citations Matilainen,
Page 155 and 156: Agilent 1260 Infinity Bio-inert Qua
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BioHPLC Column Selection Guide Cover - Agilent Technologies

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