08.11.2012 Views

BioHPLC Column Selection Guide Cover - Agilent Technologies

BioHPLC Column Selection Guide Cover - Agilent Technologies

BioHPLC Column Selection Guide Cover - Agilent Technologies

SHOW MORE
SHOW LESS

You also want an ePaper? Increase the reach of your titles

YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.

2-D LC/MS Analyses Using ZORBAX Capillary<br />

and Nano LC <strong>Column</strong>s<br />

Typical <strong>Column</strong> Configuration for 2-D HPLC<br />

1100/1200 Series<br />

micro well plate<br />

autosampler<br />

1100/1200 Series<br />

micro well plate<br />

autosampler<br />

SCX Strong<br />

cation-exchange column<br />

1100/1200 Series<br />

quaternary pump<br />

Waste<br />

1100/1200 Series<br />

quaternary pump<br />

Waste<br />

Capillary and Nano <strong>Column</strong>s<br />

Waste<br />

Reversed-phase column 1100/1200<br />

Series column compartment<br />

Waste<br />

Reversed-phase column 1100/1200<br />

Series column compartment<br />

Reversed-phase<br />

enrichment column<br />

Nanopump<br />

Detection<br />

(1100/1200 Series<br />

LC/MSD Trap)<br />

Reversed-phase<br />

enrichment column<br />

Nanopump<br />

Detection<br />

(1100/1200 Series<br />

LC/MSD Trap)<br />

Flow path of the <strong>Agilent</strong> 1100 Series Nanoflow Proteomics Solution system.<br />

1. Sample loading, elution from SCX and trapping on enrichment column<br />

2. Valve switch in column compartment, elution from enrichment column; separation on RP, and MS anaylsis<br />

ANALYSIS SCALE<br />

111

Hooray! Your file is uploaded and ready to be published.

Saved successfully!

Ooh no, something went wrong!