BioHPLC Column Selection Guide Cover - Agilent Technologies

Analysis of representative whey proteins
Column: PL-SAX 1000Å
PL1551-1802
4.6 x 50 mm, 8 µm
Mobile Phase: A: 0.02 M Tris HCI, pH 7
B: A + 0.5 M CH3COONa, pH 7
Flow Rate: 1.0 mL/min
Gradient: Linear 0-50% B in 10 min
Detector: UV, 280 nm
High resolution separation of
a Poly-T-Oligonucleotide size standard
spiked with 10mer, 15mer, 30mer
and 50mer (main peaks)
Column: PL-SAX 1000Å
PL1551-1802
4.6 x 50 mm, 8 µm
Mobile Phase: A: 7:93 v/v ACN: 0.1 M TEAA, pH 8.5
B: 7:93 v/v ACN: 0.1 M TEAA,
1 M ammonium chloride, pH 8.5
Gradient: 0-40% B in 10 min, followed by 40-70% B
in 14 min and 70-100% B in 25 min
Flow Rate: 1.5 mL/min
Temperature: 60 °C
Detector: UV, 220 nm
2000
0
0
1
0
2
3
4
ion-exchange Chromatography
min 18
10
15
1. Carbonic anhydrase
2. α-lactalbumin
3. β-lactoglobulin B
4. β-lactoglobulin A
30 50
min 50
VLC0045
UHPLC/HPLC
5