BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
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Standard protein separation<br />
<strong>Column</strong>: PL-SCX 1000Å<br />
PL1545-1502<br />
4.6 x 50 mm, 5 µm<br />
Mobile Phase: A: 20 mM KH2PO4, pH 6.0<br />
B: A + 1 M NaCl<br />
Gradient: 0-100% B in 20 min<br />
Flow Rate: 1.0 mL/min<br />
Detector: UV, 280 nm<br />
PL-SCX Strong Cation-Exchange <strong>Column</strong>s<br />
• Optimal design for effective separation of biomolecules<br />
• Pore sizes allow use of a range of solute sizes<br />
• Exceptional stability for long column lifetime<br />
ion-exchange Chromatography<br />
PL-SCX -SO 3 - is a macroporous PS/DVB matrix with a very hydrophilic coating and strong cationexchange<br />
functionality. This process is controlled to provide the optimum density of strong cationexchange<br />
moieties for the analysis, separation and purification of a wide range of biomolecules, from<br />
small peptides to large proteins. Two pore sizes are available, 1000Å and 4000Å, to provide good<br />
mass transfer characteristics for a range of solute sizes. The 5 µm media delivers separations at<br />
higher resolution with the 30 µm media used for medium pressure liquid chromatography.<br />
<strong>Column</strong> Specifications<br />
Bonded Phase ID Particle Size Pore Size pH Stability<br />
Operating<br />
Temperature<br />
Limit<br />
Strong Cation-Exchange 2.1, 4.6, 5, 8, 10 1000Å 1-14 80 ºC<br />
7.5, 25, 50<br />
and 100<br />
and 30 and 4000Å<br />
0<br />
1<br />
2<br />
3<br />
min<br />
4<br />
1. Myoglobin<br />
2. Chymotrypsinogen A<br />
3. Cytochrome c<br />
4. Lysozyme<br />
25<br />
VLC0046<br />
UHPLC/HPLC<br />
59
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