BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
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<strong>Column</strong> Specifications<br />
<strong>Agilent</strong> Bio SEC-5<br />
size exclusion Chromatography (seC)<br />
• Maximum recovery for a broad range of size-based, biomolecule separations<br />
• Outstanding reproducibility and column lifetime<br />
• Excellent stability, even under high-pH, high-salt, and low-salt conditions<br />
• Compatibility with most aqueous buffers<br />
<strong>Agilent</strong> Bio SEC-5 HPLC columns are packed with 5 µm silica particles coated with a proprietary,<br />
neutral, hydrophilic layer for maximum efficiency and stability. Our specially designed packing also<br />
provides high pore volume, improving both peak capacity and resolution.<br />
Bio SEC-5 columns are available in 5 µm particles with 100Å, 150Å, 300Å, 500Å, 1000Å,<br />
and 2000Å nominal pore sizes.<br />
Pore Size Particle Size MW Range pH Range Max Pressure Flow Rate<br />
100Å 5 µm 100-100,000 2-8.5 240 bar, 3500 psi 0.1-1.25 mL/min (7.8 mm id)<br />
0.1-0.4 mL/min (4.6 mm id)<br />
150Å 5 µm 500-150,000 2-8.5 240 bar, 3500 psi 0.1-1.25 mL/min (7.8 mm id)<br />
0.1-0.4 mL/min (4.6 mm id)<br />
300Å 5 µm 5,000-1,250,000 2-8.5 240 bar, 3500 psi 0.1-1.25 mL/min (7.8 mm id)<br />
0.1-0.4 mL/min (4.6 mm id)<br />
500Å 5 µm 15,000-5,000,000 2-8.5 240 bar, 3500 psi 0.1-1.25 mL/min (7.8 mm id)<br />
0.1-0.4 mL/min (4.6 mm id)<br />
1000Å 5 µm 50,000-7,500,000 2-8.5 240 bar, 3500 psi 0.1-1.25 mL/min (7.8 mm id)<br />
0.1-0.4 mL/min (4.6 mm id)<br />
2000Å 5 µm >10,000,000 2-8.5 240 bar, 3500 psi 0.1-1.25 mL/min (7.8 mm id)<br />
0.1-0.4 mL/min (4.6 mm id)<br />
UHPLC/HPLC<br />
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