BioHPLC Column Selection Guide Cover - Agilent Technologies

affinity Chromatography
LC Column Reagent Starter Kit, 5185-5986
Luer-Lok adapters, 5188-5249
Luer-Lok syringe, 5188-5250
Luer-Lok needles, 5188-5253
9 www.agilent.com/chem/biohplc
Multiple Affinity Removal System Starter Kits
The LC Column and Spin Cartridge Reagent Starter Kits include all the required supplies to use with
Multiple Affinity Removal System. These buffers provide optimal conditions for column longevity and
sample reproducibility.
• The kits provide enough Buffer A and Buffer B for approximately 200 sample depletions using
the 4.6 x 50 mm LC columns, approximately 100 sample depletions using the 4.6 x 100 mm LC
columns and 200 spin cartridge uses.
• Buffer A, the loading buffer, minimizes protein-protein interactions, allowing low abundant
proteins often bound to high abundant proteins to pass through the column, while the targeted
high abundant proteins bind to their associated antibodies.
• Buffer B, the elution buffer, then disrupts the antibody-protein interaction eluting the high
abundant proteins off the column.
Multiple Affinity Removal System Starter Kits
Description Part No.
LC Column Reagent Starter Kit
Includes:
5185-5986
Buffer A, 1 L, for loading, washing, and equilibrating 5185-5987
Buffer B, 1 L, for eluting 5185-5988
Spin filters, 0.22 µm cellulose acetate, 25/pk 5185-5990
Spin concentrators, 5K MWCO, 4 mL, 25/pk 5185-5991
Mult Aff Rem Spin Cartridge Reagent Kit
Includes:
5188-5254
Buffer A, 1 L, for loading, washing, and equilibrating 5185-5987
Buffer B, 1 L, for eluting 5185-5988
Spin filters, 0.22 µm cellulose acetate, 25/pk 5185-5990
Spin concentrators, 5K MWCO, 4 mL, 25/pk 5185-5991
Luer-Lok adapters, 2/pk 5188-5249
Plastic syringe, 5 mL, Luer-Lok, 2/pk 5188-5250
Microtube, 1.5 mL, screw top, 100/pk 5188-5251
Caps and plugs, 6/pk 5188-5252
PTFE needles, Luer-Lok, 10/pk 5188-5253
High concentration sample dilution buffer, 50 mL, 5188-8283