BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
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Retention volume versus log (MW)<br />
for the Bio-Rad standards separated<br />
on an <strong>Agilent</strong> ZORBAX GF-250 column<br />
<strong>Column</strong>: ZORBAX GF-250<br />
884973-901<br />
9.4 x 250 mm, 4 µm<br />
Mobile Phase: 200 mM Sodium phosphate, pH 7.0<br />
Temperature: Ambient<br />
Detector: UV (254 nm)<br />
Separations of proteins on preparative columns<br />
<strong>Column</strong> A: ZORBAX GF-250<br />
<strong>Column</strong> B: ZORBAX GF-450<br />
Mobile Phase: 0.2 M Na 2HPO 4, pH 7.0<br />
Flow Rate: 5.0 mL/min<br />
Detector: 280 nm<br />
Sample: 200 µL<br />
Log (MW)<br />
ZORBAX GF-250<br />
0<br />
ZORBAX GF-450<br />
size exclusion Chromatography (seC)<br />
Thyroglobulin (670 Kdal)<br />
Gamma globulin (158 Kdal)<br />
Ovalbumin (44 Kdal)<br />
Myoglobulin (17 Kdal)<br />
1<br />
1<br />
2<br />
Vitamin B 12 (1.35 Kdal)<br />
Retention volume (mL)<br />
3 4<br />
5 6<br />
5 10 15<br />
5<br />
3 4<br />
2<br />
0 5 10<br />
Retention time (min)<br />
15<br />
6<br />
1. Thyroglobulin<br />
2. Catalase<br />
3. BSA<br />
4. ß-Lactoglobulin B<br />
5. Myoglobin<br />
6. Tyr-Gly-Gly<br />
UHPLC/HPLC
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