BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
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<strong>Column</strong> <strong>Selection</strong> Flow Chart<br />
Biocolumn <strong>Selection</strong> <strong>Guide</strong>lines<br />
The flow chart below indicates the page numbers that will take you to the selection guides in the individual chapters that will help you choose the best<br />
column for your biomolecule application.<br />
There are a number of guidelines that can be followed to assist in the selection of the optimum column for a biomolecule separation. The starting point<br />
will be the size of the molecule, as this will determine the pore size of the HPLC method used for the separation. Secondly, consider the solubility of the<br />
molecule. Thirdly, note the separation mechanism, size, hydrophobicity, and charge.<br />
Biomolecule<br />
Primary Structure<br />
Aggregates<br />
Fragment Pattern<br />
Charge Isoforms<br />
Affinity<br />
Reversed-Phase<br />
Size Exclusion<br />
Chromatography (SEC)<br />
Ion-Exchange (IEX)<br />
Protein A<br />
See page 17<br />
See page 65<br />
See page 46<br />
See page 89<br />
<strong>BioHPLC</strong> COLUMNS<br />
3
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