BioHPLC Column Selection Guide Cover - Agilent Technologies

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size exclusion Chromatography (seC) 2 www.agilent.com/chem/biohplc Agilent Bio SEC-3 Size (mm) Particle Size (µm) Bio SEC-3 100Å USP L33 Bio SEC-3 150Å USP L33 Bio SEC-3 300Å USP L33 7.8 x 300 3 5190-2501 5190-2506 5190-2511 7.8 x 150 3 5190-2502 5190-2507 5190-2512 4.6 x 300 3 5190-2503 5190-2508 5190-2513 4.6 x 150 3 5190-2504 5190-2509 5190-2514 7.8 x 50, Guard 3 5190-2505 5190-2510 5190-2515
Column Specifications Agilent Bio SEC-5 size exclusion Chromatography (seC) • Maximum recovery for a broad range of size-based, biomolecule separations • Outstanding reproducibility and column lifetime • Excellent stability, even under high-pH, high-salt, and low-salt conditions • Compatibility with most aqueous buffers Agilent Bio SEC-5 HPLC columns are packed with 5 µm silica particles coated with a proprietary, neutral, hydrophilic layer for maximum efficiency and stability. Our specially designed packing also provides high pore volume, improving both peak capacity and resolution. Bio SEC-5 columns are available in 5 µm particles with 100Å, 150Å, 300Å, 500Å, 1000Å, and 2000Å nominal pore sizes. Pore Size Particle Size MW Range pH Range Max Pressure Flow Rate 100Å 5 µm 100-100,000 2-8.5 240 bar, 3500 psi 0.1-1.25 mL/min (7.8 mm id) 0.1-0.4 mL/min (4.6 mm id) 150Å 5 µm 500-150,000 2-8.5 240 bar, 3500 psi 0.1-1.25 mL/min (7.8 mm id) 0.1-0.4 mL/min (4.6 mm id) 300Å 5 µm 5,000-1,250,000 2-8.5 240 bar, 3500 psi 0.1-1.25 mL/min (7.8 mm id) 0.1-0.4 mL/min (4.6 mm id) 500Å 5 µm 15,000-5,000,000 2-8.5 240 bar, 3500 psi 0.1-1.25 mL/min (7.8 mm id) 0.1-0.4 mL/min (4.6 mm id) 1000Å 5 µm 50,000-7,500,000 2-8.5 240 bar, 3500 psi 0.1-1.25 mL/min (7.8 mm id) 0.1-0.4 mL/min (4.6 mm id) 2000Å 5 µm >10,000,000 2-8.5 240 bar, 3500 psi 0.1-1.25 mL/min (7.8 mm id) 0.1-0.4 mL/min (4.6 mm id) UHPLC/HPLC 3
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Agilent BioHPLC Column Selection Gu
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BIOCOLUMN SELECTION GUIDELINES From
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Column Selection Flow Chart Biocolu
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Protein Column Selection Guide Appl
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Separation of charge variants of hu
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Increased resolution for peptide ma
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Preparative scale purification of L
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DNA and RNA Oligonucleotide Column
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Broad Distribution Biomolecules Car
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ReveRseD-PHase HPLC Confidently per
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Sterically Protected 300StableBond
Page 23 and 24: Improved reproducibility of monoclo
Page 25 and 26: Short-chain ZORBAX 300SB-C3 is stab
Page 27 and 28: ZORBAX 300Å StableBond Hardware De
Page 29 and 30: LC/MS analysis of angiotensin on Ex
Page 31 and 32: Tips & Tools ZORBAX 300Å Extend-C1
Page 33 and 34: Poroshell 300 columns separate prot
Page 35 and 36: Monoclonal IgG1 chains: Separation
Page 37 and 38: Poroshell 120 • 120Å pore size f
Page 39 and 40: 0 µm 0 µm 0 µm A B C Scanning el
Page 41 and 42: Exploiting chemical stability - TFA
Page 43 and 44: Temperature as a tool to enhance ma
Page 45 and 46: ZORBaX amino acid analysis (aaa) Co
Page 47 and 48: High resolution of 24 amino acids u
Page 49 and 50: Ion-Exchange Column Selection Appli
Page 51 and 52: Consistent ion-exchange MAb separat
Page 53 and 54: Column Specifications Tips & Tools
Page 55 and 56: Weak cation-exchange chromatography
Page 57 and 58: PL-SAX Strong Anion-Exchange Column
Page 59 and 60: Analysis of representative whey pro
Page 61 and 62: Standard protein separation Column:
Page 63 and 64: Agilent Bio-Monolith HPLC Column Se
Page 65 and 66: Baseline expansion of a separation
Page 67 and 68: size exclusion Chromatography (seC)
Page 69 and 70: Column Specifications Agilent Bio S
Page 71 and 72: Comparison of Agilent Bio SEC-3 and
Page 73: Column Length size exclusion Chroma
Page 77 and 78: Calibration curves - Bio SEC-5 Colu
Page 79 and 80: ProSEC 300S • Mechanically robust
Page 81 and 82: Overlay of UV and light scattering
Page 83 and 84: Retention volume versus log (MW) fo
Page 85 and 86: Agilent PL aquagel-OH SEC Columns f
Page 87 and 88: Heparin Column: 2 x PL aquagel-OH 3
Page 89 and 90: Polyethylene Glycol/Oxide standards
Page 91 and 92: aFFiniTY CHROMaTOGRaPHY Agilent Bio
Page 93 and 94: Rapid human polyclonal IgG quantifi
Page 95 and 96: Multiple Affinity Removal System af
Page 97 and 98: Illustration of high abundance prot
Page 99 and 100: mRP-C18 High-Recovery Protein Colum
Page 101 and 102: MeTHOD DeveLOPMenT ZORBAX Column Me
Page 103 and 104: Reversed-Phase LC/MS Methods Method
Page 105 and 106: Method Development Ionic Strength:
Page 107 and 108: Method Development After the initia
Page 109 and 110: Tips & Tools Agilent offers a varie
Page 111 and 112: Human serum: Low abundance protein
Page 113 and 114: 2-D LC/MS Analyses Using ZORBAX Cap
Page 115 and 116: ZORBAX Bio-SCX Series II Capillary
Page 117 and 118: ZORBAX HPLC Capillary Columns (glas
Page 119 and 120: Microbore HPLC for sensitive peptid
Page 121 and 122: BioPharmaceutical Lifecycle Reverse
Page 123 and 124: ZORBAX 300Å StableBond ZORBAX Prep
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PLRP-S Prep to Process Application
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Prep to Process PLRP-S Size (mm) Pa
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PL-SAX AND PL-SCX FOR PREP TO PROCE
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Prep to Process PL-SAX and PL-SCX D
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Crude peptide screen Column: VariTi
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CARTRIDGE COLUMN SYSTEMS Cartridge
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USP DESIGNATIONS - BIOHPLC COLUMNS
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BioHPLC Columns Literature Title Ag
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BioHPLC Columns Literature Title An
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BioHPLC Columns Literature Title Co
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BIOPHARM DEFINITIONS A active start
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dimer A polymer made up of two iden
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K-L ligase An enzyme that causes mo
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S secondary structure In proteins,
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Poroshell 300 Citations Matilainen,
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Agilent 1260 Infinity Bio-inert Qua
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BioHPLC Column Selection Guide Cover - Agilent Technologies

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