BioHPLC Column Selection Guide Cover - Agilent Technologies

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affinity Chromatography Multiple Affinity Removal System Selection Guide Product Proteins Removed MARS Human-14 Albumin, IgG, antitrypsin, IgA, transferrin, haptoglobin, fibrinogen, alpha2macroglobulin, alpha1-acid glycoprotein, IgM, apolipoprotein Al, apolipoprotein All, complement C3, transthyretin MARS Human-7 Albumin, IgG, IgA, transferrin, haptoglobin, antitrypsin, fibrinogen MARS Human-6 Albumin, IgG, IgA, transferrin, haptoglobin, antitrypsin MARS Human-6 High Capacity Albumin, IgG, IgA, transferrin, haptoglobin, antitrypsin 94 www.agilent.com/chem/biohplc Total Protein Removed Dimension Load Capacity Part No. 94% Spin Cartridge 8 - 10 µL 5188-6560 4.6 x 50 mm 20 µL 5188-6557 4.6 x 100 mm 40 µL 5188-6558 10 x 100 mm 250 µL 5188-6559 88-92% Spin Cartridge 12 - 14 µL 5188-6408 4.6 x 50 mm 30 - 35 µL 5188-6409 4.6 x 100 mm 60 - 70 µL 5188-6410 10 x 100 mm 250 - 300 µL 5188-6411 85-90% Spin Cartridge 7 - 10 µL 5188-5230 4.6 x 50 mm 15 - 20 µL 5185-5984 4.6 x 100 mm 30 - 40 µL 5185-5985 85-90% Spin Cartridge 14 - 16 µL 5188-5341 4.6 x 50 mm 30 - 40 µL 5188-5332 4.6 x 100 mm 60 - 80 µL 5188-5333 10 x 100 mm up to 340 µL 5188-5336 MARS Human-2 Albumin, IgG 69% Spin Cartridge 50 µL 5188-8825 4.6 x 50 mm 100 µL 5188-8826 MARS Human-1 Albumin 50-55% Spin Cartridge 65 µL 5188-5334 4.6 x 50 mm 130 µL 5188-6562 MARS Mouse-3 Albumin, IgG, transferrin 80% Spin Cartridge 25 - 30 µL 5188-5289 4.6 x 50 mm 37 - 50 µL 5188-5217 4.6 x 100 mm 75 - 100 µL 5188-5218
Illustration of high abundance proteins removed by Agilent Multiple Affinity Removal Columns and Spin Cartridges IgG Albumin 15% Transferrin Fibrinogen IgA Haptoglobin α1-Antitrypsin Complement C3 α1-Acid Glycoprotein α2-Macroglobulin Apolipoprotein AI Apolipoprotein AII IgM Transthyretin affinity Chromatography 6% Remaining for Analysis UHPLC/HPLC 95
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Agilent BioHPLC Column Selection Gu
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BIOCOLUMN SELECTION GUIDELINES From
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Column Selection Flow Chart Biocolu
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Protein Column Selection Guide Appl
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Separation of charge variants of hu
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Increased resolution for peptide ma
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Preparative scale purification of L
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DNA and RNA Oligonucleotide Column
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Broad Distribution Biomolecules Car
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ReveRseD-PHase HPLC Confidently per
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Sterically Protected 300StableBond
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Improved reproducibility of monoclo
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Short-chain ZORBAX 300SB-C3 is stab
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ZORBAX 300Å StableBond Hardware De
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LC/MS analysis of angiotensin on Ex
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Tips & Tools ZORBAX 300Å Extend-C1
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Poroshell 300 columns separate prot
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Monoclonal IgG1 chains: Separation
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Poroshell 120 • 120Å pore size f
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0 µm 0 µm 0 µm A B C Scanning el
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Exploiting chemical stability - TFA
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Temperature as a tool to enhance ma
Page 45 and 46: ZORBaX amino acid analysis (aaa) Co
Page 47 and 48: High resolution of 24 amino acids u
Page 49 and 50: Ion-Exchange Column Selection Appli
Page 51 and 52: Consistent ion-exchange MAb separat
Page 53 and 54: Column Specifications Tips & Tools
Page 55 and 56: Weak cation-exchange chromatography
Page 57 and 58: PL-SAX Strong Anion-Exchange Column
Page 59 and 60: Analysis of representative whey pro
Page 61 and 62: Standard protein separation Column:
Page 63 and 64: Agilent Bio-Monolith HPLC Column Se
Page 65 and 66: Baseline expansion of a separation
Page 67 and 68: size exclusion Chromatography (seC)
Page 69 and 70: Column Specifications Agilent Bio S
Page 71 and 72: Comparison of Agilent Bio SEC-3 and
Page 73 and 74: Column Length size exclusion Chroma
Page 75 and 76: Column Specifications Agilent Bio S
Page 77 and 78: Calibration curves - Bio SEC-5 Colu
Page 79 and 80: ProSEC 300S • Mechanically robust
Page 81 and 82: Overlay of UV and light scattering
Page 83 and 84: Retention volume versus log (MW) fo
Page 85 and 86: Agilent PL aquagel-OH SEC Columns f
Page 87 and 88: Heparin Column: 2 x PL aquagel-OH 3
Page 89 and 90: Polyethylene Glycol/Oxide standards
Page 91 and 92: aFFiniTY CHROMaTOGRaPHY Agilent Bio
Page 93 and 94: Rapid human polyclonal IgG quantifi
Page 95: Multiple Affinity Removal System af
Page 99 and 100: mRP-C18 High-Recovery Protein Colum
Page 101 and 102: MeTHOD DeveLOPMenT ZORBAX Column Me
Page 103 and 104: Reversed-Phase LC/MS Methods Method
Page 105 and 106: Method Development Ionic Strength:
Page 107 and 108: Method Development After the initia
Page 109 and 110: Tips & Tools Agilent offers a varie
Page 111 and 112: Human serum: Low abundance protein
Page 113 and 114: 2-D LC/MS Analyses Using ZORBAX Cap
Page 115 and 116: ZORBAX Bio-SCX Series II Capillary
Page 117 and 118: ZORBAX HPLC Capillary Columns (glas
Page 119 and 120: Microbore HPLC for sensitive peptid
Page 121 and 122: BioPharmaceutical Lifecycle Reverse
Page 123 and 124: ZORBAX 300Å StableBond ZORBAX Prep
Page 125 and 126: PLRP-S Prep to Process Application
Page 127 and 128: Prep to Process PLRP-S Size (mm) Pa
Page 129 and 130: PL-SAX AND PL-SCX FOR PREP TO PROCE
Page 131 and 132: Prep to Process PL-SAX and PL-SCX D
Page 133 and 134: Crude peptide screen Column: VariTi
Page 135 and 136: CARTRIDGE COLUMN SYSTEMS Cartridge
Page 137 and 138: USP DESIGNATIONS - BIOHPLC COLUMNS
Page 139 and 140: BioHPLC Columns Literature Title Ag
Page 141 and 142: BioHPLC Columns Literature Title An
Page 143 and 144: BioHPLC Columns Literature Title Co
Page 145 and 146: BIOPHARM DEFINITIONS A active start
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dimer A polymer made up of two iden
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K-L ligase An enzyme that causes mo
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S secondary structure In proteins,
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Poroshell 300 Citations Matilainen,
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Agilent 1260 Infinity Bio-inert Qua
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BioHPLC Column Selection Guide Cover - Agilent Technologies

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