BioHPLC Column Selection Guide Cover - Agilent Technologies

More documents

Recommendations

Info

Prep to Process PL-SAX and PL-SCX Purification of a large oligonucleotide Column: PL-SAX 1000Å, 8 µm Mobile Phase: A: 93% 0.1 M TEAA, pH 7:7% ACN B: 93% 0.1 M TEAA, 3.24 M ammonium acetate, pH 7:7% ACN Gradient: 0-100% B in 20 min Flow Rate: 1.5 mL/min Temperature: 60 °C Detector: UV, 290 nm Preparative fractionation of a culture filtrate containing amyloglucosidases on Agilent PL-SAX 4000Å Column: PL-SAX PL1551-1803 4.6 x 50 mm, 8 µm Mobile Phase: A: 0.01 M Tris HCI, pH 8 B: A + 0.5 M NaCl, pH 8 Flow Rate: 4.0 mL/min Gradient: Linear 0-100% B in 2 min Detector: UV, 280 nm 128 www.agilent.com/chem/biohplc Column Specifications PL-SAX PL-SCX Matrix Fully polymeric Fully polymeric Pore Sizes 1000Å, 4000Å 1000Å, 4000Å Particle Sizes 10 µm, 30 µm 10 µm, 30 µm Bead Form Rigid spherical Rigid spherical Functionality Quaternary amine Sulfonic acid Pressure Stability 3000 psi 3000 psi Temperature Stability 80 °C 80 °C pH Range 1-14 1-14 Eluent Compatibility All anion-exchange buffers All cation-exchange buffers Packed Bed Density 0.39 g/mL 0.39 g/mL 80 %B 0 0 min Preparative fraction 91 mer 35 VLC0054 0 min 30
Prep to Process PL-SAX and PL-SCX Dimensions Particle Size (µm) Prep to Process PL-SAX and PL-SCX PL-SAX 1000Å PL-SAX 4000Å PL-SCX 1000Å PL-SCX 4000Å 100 x 300 30 PL1851-3102 PL1851-3103 PL1845-3102 PL1845-3103 100 x 300 10 PL1851-2102 PL1851-2103 PL1845-2102 PL1845-2103 50 x 150 30 PL1751-3702 PL1751-3703 PL1745-3702 PL1745-3703 50 x 150 10 PL1751-3102 PL1751-3103 PL1745-3102 PL1745-3103 25 x 150 30 PL1251-3702 PL1251-3703 PL1245-3702 PL1245-3703 25 x 150 10 PL1251-3102 PL1251-3103 PL1245-3102 PL1245-3103 25 x 50 10 PL1251-1102 PL1251-1103 PL1245-1102 PL1245-1103 7.5 x 150 8 PL1151-3802 PL1151-3803 7.5 x 50 8 PL1151-1802 PL1151-1803 PL1145-1802 PL1145-1803 PL-SAX and PL-SCX Method Development Columns 4.6 x 250 30 PL1551-5702 PL1551-5703 PL1545-5702 PL1545-5703 4.6 x 250 10 PL1551-5102 PL1551-5103 PL1545-5102 PL1545-5103 4.6 x 150 30 PL1551-3702 PL1551-3703 PL1545-3702 PL1545-3703 4.6 x 150 10 PL1551-3102 PL1551-3103 PL1545-3102 PL1545-3103 PL-SAX and PL-SCX Bulk Media Particle Size (µm) Unit PL-SAX 1000Å PL-SAX 4000Å PL-SCX 1000Å PL-SCX 4000Å 30 1 kg PL1451-6702 PL1451-6703 PL1445-6702 PL1445-6703 100 g PL1451-4702 PL1451-4703 PL1445-4702 PL1445-4703 10 1 kg PL1451-6102 PL1451-6103 PL1445-6102 PL1445-6103 100 g PL1451-4102 PL1451-4103 PL1445-4102 PL1445-4103 For larger quantities, please contact your local Agilent sales office. PURIFICATION 129
Page 1 and 2:
Agilent BioHPLC Column Selection Gu
Page 3 and 4:
BIOCOLUMN SELECTION GUIDELINES From
Page 5 and 6:
Column Selection Flow Chart Biocolu
Page 7 and 8:
Protein Column Selection Guide Appl
Page 9 and 10:
Separation of charge variants of hu
Page 11 and 12:
Increased resolution for peptide ma
Page 13 and 14:
Preparative scale purification of L
Page 15 and 16:
DNA and RNA Oligonucleotide Column
Page 17 and 18:
Broad Distribution Biomolecules Car
Page 19 and 20:
ReveRseD-PHase HPLC Confidently per
Page 21 and 22:
Sterically Protected 300StableBond
Page 23 and 24:
Improved reproducibility of monoclo
Page 25 and 26:
Short-chain ZORBAX 300SB-C3 is stab
Page 27 and 28:
ZORBAX 300Å StableBond Hardware De
Page 29 and 30:
LC/MS analysis of angiotensin on Ex
Page 31 and 32:
Tips & Tools ZORBAX 300Å Extend-C1
Page 33 and 34:
Poroshell 300 columns separate prot
Page 35 and 36:
Monoclonal IgG1 chains: Separation
Page 37 and 38:
Poroshell 120 • 120Å pore size f
Page 39 and 40:
0 µm 0 µm 0 µm A B C Scanning el
Page 41 and 42:
Exploiting chemical stability - TFA
Page 43 and 44:
Temperature as a tool to enhance ma
Page 45 and 46:
ZORBaX amino acid analysis (aaa) Co
Page 47 and 48:
High resolution of 24 amino acids u
Page 49 and 50:
Ion-Exchange Column Selection Appli
Page 51 and 52:
Consistent ion-exchange MAb separat
Page 53 and 54:
Column Specifications Tips & Tools
Page 55 and 56:
Weak cation-exchange chromatography
Page 57 and 58:
PL-SAX Strong Anion-Exchange Column
Page 59 and 60:
Analysis of representative whey pro
Page 61 and 62:
Standard protein separation Column:
Page 63 and 64:
Agilent Bio-Monolith HPLC Column Se
Page 65 and 66:
Baseline expansion of a separation
Page 67 and 68:
size exclusion Chromatography (seC)
Page 69 and 70:
Column Specifications Agilent Bio S
Page 71 and 72:
Comparison of Agilent Bio SEC-3 and
Page 73 and 74:
Column Length size exclusion Chroma
Page 75 and 76:
Column Specifications Agilent Bio S
Page 77 and 78:
Calibration curves - Bio SEC-5 Colu
Page 79 and 80: ProSEC 300S • Mechanically robust
Page 81 and 82: Overlay of UV and light scattering
Page 83 and 84: Retention volume versus log (MW) fo
Page 85 and 86: Agilent PL aquagel-OH SEC Columns f
Page 87 and 88: Heparin Column: 2 x PL aquagel-OH 3
Page 89 and 90: Polyethylene Glycol/Oxide standards
Page 91 and 92: aFFiniTY CHROMaTOGRaPHY Agilent Bio
Page 93 and 94: Rapid human polyclonal IgG quantifi
Page 95 and 96: Multiple Affinity Removal System af
Page 97 and 98: Illustration of high abundance prot
Page 99 and 100: mRP-C18 High-Recovery Protein Colum
Page 101 and 102: MeTHOD DeveLOPMenT ZORBAX Column Me
Page 103 and 104: Reversed-Phase LC/MS Methods Method
Page 105 and 106: Method Development Ionic Strength:
Page 107 and 108: Method Development After the initia
Page 109 and 110: Tips & Tools Agilent offers a varie
Page 111 and 112: Human serum: Low abundance protein
Page 113 and 114: 2-D LC/MS Analyses Using ZORBAX Cap
Page 115 and 116: ZORBAX Bio-SCX Series II Capillary
Page 117 and 118: ZORBAX HPLC Capillary Columns (glas
Page 119 and 120: Microbore HPLC for sensitive peptid
Page 121 and 122: BioPharmaceutical Lifecycle Reverse
Page 123 and 124: ZORBAX 300Å StableBond ZORBAX Prep
Page 125 and 126: PLRP-S Prep to Process Application
Page 127 and 128: Prep to Process PLRP-S Size (mm) Pa
Page 129: PL-SAX AND PL-SCX FOR PREP TO PROCE
Page 133 and 134: Crude peptide screen Column: VariTi
Page 135 and 136: CARTRIDGE COLUMN SYSTEMS Cartridge
Page 137 and 138: USP DESIGNATIONS - BIOHPLC COLUMNS
Page 139 and 140: BioHPLC Columns Literature Title Ag
Page 141 and 142: BioHPLC Columns Literature Title An
Page 143 and 144: BioHPLC Columns Literature Title Co
Page 145 and 146: BIOPHARM DEFINITIONS A active start
Page 147 and 148: dimer A polymer made up of two iden
Page 149 and 150: K-L ligase An enzyme that causes mo
Page 151 and 152: S secondary structure In proteins,
Page 153 and 154: Poroshell 300 Citations Matilainen,
Page 155 and 156: Agilent 1260 Infinity Bio-inert Qua
show all

BioHPLC Column Selection Guide Cover - Agilent Technologies

Create successful ePaper yourself

Delete template?

Save as template?