BioHPLC Column Selection Guide Cover - Agilent Technologies

Prep to Process PLRP-S
122 www.agilent.com/chem/biohplc
PLRP-S FOR REVERSED-PHASE
PREP TO PROCESS
• Discovery stage to multi-kg cGMP production reduces method development time
• Chemical stability for separations, optimization, sanitation, and regeneration increases selectivity
and column lifetime
• Single batch packing of multiple columns reduces system downtime and validation costs
The PLRP-S media, rigid poly(styrene/divinylbenzene) particles, are available in a range of pore sizes
for small molecule, synthetic biomolecule and macromolecule purification. Their thermal and chemical
stability makes them ideal for purifications that require extreme conditions for sample preparation,
compound elution, and column regeneration.
Capacity and resolution are two key parameters for maximizing the throughput of a purification.
With a wide choice of pore sizes and extended range of operating conditions, PLRP-S provides
more options to achieve the optimum process. Particle sizes range from 3 µm to 50 µm for scale-up
from the µg/mg discovery stage to multi-kg cGMP production. Excellent chemical stability, up to
1 M NaOH, permits sanitation and regeneration that increase column lifetime. PLRP-S media batch
sizes of up to 600 L are available, providing single batch packing of multiple columns.
As part of our commitment to quality and continuity of supply, all manufacturing is carried out under
a fully documented process. A Type II Drug Master File and regulatory support files are available
for process materials, and facility audits are routinely conducted.