BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
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size exclusion Chromatography (seC)<br />
<strong>Column</strong> Specifications<br />
0 www.agilent.com/chem/biohplc<br />
ZORBAX GF-250 and GF-450 Gel Filtration <strong>Column</strong>s<br />
• High efficiency and reproducibility with short analysis time<br />
• Semi-prep and prep column dimensions<br />
• Compatible with organic modifiers and denaturants<br />
• Wide usable pH range (3-8)<br />
<strong>Agilent</strong> ZORBAX GF-250 and GF-450 size exclusion (gel filtration) columns are ideal for size<br />
separations of proteins and other biomolecules. The separation range is 4,000-900,000 for globular<br />
proteins when using GF-250 and GF-450 columns in series. The GF-250/GF-450 size exclusion<br />
columns have a hydrophilic diol bonded phase for high recovery of proteins (typically >90%)<br />
and a unique zirconia modification of the silica for a pH operating range from 3-8. The GF-250<br />
and GF-450 columns are packed with precisely sized porous silica microspheres with narrow pore<br />
size and particle size distributions. The result is a highly efficient, rugged and reproducible size<br />
exclusion column that can be used for both analytical and preparative separations of proteins with<br />
flow rates of up to 3 mL/min. These columns are compatible with organic modifiers (
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