BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
BioHPLC Column Selection Guide Cover - Agilent Technologies
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Prep to Process PLRP-S<br />
Purification of a 25-mer trityl-off oligonucleotide<br />
and analytical quantitation of the fraction using<br />
PLRP-S 100Å, 4.6 x 50 mm<br />
Crude bradykinin prep load<br />
<strong>Column</strong>: PLRP-S 100Å<br />
PL1512-5100<br />
4.6 x 250 mm, 10 µm<br />
Sample: 30 µL containing 1.5 mg of crude peptide<br />
Mobile Phase: 0.1% TFA in 21% ACN:79% water<br />
Flow Rate: 1 mL/min (360 cm/hr)<br />
Fraction analysis –<br />
the concentration<br />
overload purification<br />
124 www.agilent.com/chem/biohplc<br />
200<br />
mV<br />
0<br />
80<br />
mV<br />
0<br />
0<br />
0<br />
min 12.5<br />
VLC0053<br />
0 min<br />
30<br />
VLCbradykinin<br />
HPLC Purity %<br />
min<br />
Fraction number<br />
Purity (CE): 90.54%<br />
Recovery: 83.20%<br />
25<br />
% Purity (Bradykinin)<br />
% Purity (Impurity)
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