Narcissus and Daffodil

mg/g DW
mg/g DW
0.90
0.75
0.60
0.45
0.30
0.15
0.00
3.50
3.00
2.50
2.00
1.50
1.00
0.50
0.00
N-formylnorgalanthamine
0 1 3
BA (mg/l)
5 10
Haemanthamine
0 1 3
BA (mg/l)
5 10
ts
cm
ts
cm
Production of galanthamine in vitro 223
mg/g DW
mg/g DW
Galanthamine
Total production of alkaloids during the experiment
The levels of alkaloids produced throughout culture are shown in Table 7.2. The
highest accumulation of alkaloids was observed in the shoot-clumps treated with
3 mg/l of BA, which also displayed the best growth, followed by those treated with
1 mg/l of BA. The absence of BA coincided with the lowest accumulation of alkaloids.
2.50
2.00
1.50
1.00
0.50
0.00
0.35
0.30
0.25
0.20
0.15
0.10
0.05
0.00
0 1 3
BA (mg/l)
5 10
Tazettine
0 1 3
BA (mg/l)
5 10
Figure 7.6 Accumulation of alkaloids in both tissue (shoot-clumps) and liquid medium at
the end of the experiment with BA. Bars represent the SD calculated from three
replicates. ts, alkaloids detected in the tissue; cm, alkaloids released to the
culture medium.
Table 7.2 Total production of alkaloids (accumulation in both tissue and liquid
medium) in shoot-clumps grown under different concentrations of BA
(µg/g FW ± SD calculated from three replicates) a
BA NFNGAL GAL HAEM TAZ
0 mg/l 284.2 ± 14.1 475.5 ± 35.1 488.8 ± 39.0 79.7 ± 5.6
1 mg/l 355.6 ± 19.4 903.7 ± 52.3 1358.5 ± 78.5 157.4 ± 8.2
3 mg/l 687.4 ± 38.7 1354.2 ± 83.3 2097.5 ± 129.1 248.7 ± 13.2
5 mg/l 347.1 ± 23.4 572.1 ± 46.9 856.1 ± 77.2 110.8 ± 6.8
10 mg/l 377.8 ± 14.3 465.7 ± 18.3 673.0 ± 30.1 115.3 ± 6.8
Note
a for abbreviations, see Table 7.1.
ts
cm
ts
cm