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Narcissus and Daffodil

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278 R.M. Moraes<br />

Galanthamine content<br />

mg /100g dry weight<br />

300<br />

250<br />

200<br />

150<br />

100<br />

50<br />

0<br />

AGRONOMIC FACTORS AFFECTING DRUG YIELD<br />

Genetic <strong>and</strong> environmental factors strongly influence biomass growth <strong>and</strong> its<br />

content of secondary compounds. To achieve maximum yields of any active compound<br />

such as galanthamine, optimum agronomic practices should be established.<br />

The agronomic practices include the identification of high-yielding cultivars,<br />

optimum growing conditions (climate, soil type, planting density, fertilisation <strong>and</strong><br />

irrigation), pest management (weeds, insects <strong>and</strong> disease), <strong>and</strong> appropriate<br />

mechanisation for cultivation, harvesting, drying <strong>and</strong> grinding.<br />

As mentioned above, a survey of more than 80 <strong>Narcissus</strong> taxa indicated that<br />

<strong>Narcissus</strong> ‘Inglescombe’ had the highest galanthamine content (Bastos et al., 1996).<br />

However, the current supply of ‘Inglescombe’ bulbs is small <strong>and</strong> requires a mass<br />

propagation programme to increase stocks for extensive cultivation.<br />

Propagation<br />

I II III IV V VI VII VIII IX X XI XII<br />

Developmental Stage<br />

Figure 10.1 The effect of developmental stages of <strong>Narcissus</strong> ‘Inglescombe’ on galanthamine<br />

content during the 1994 growing season (unpublished data):<br />

I, pre-emergence (1 February); II, emergence, pre-bud stage (26 February);<br />

III, post-emergence, early bud stage (10 March); IV, anthesis (23 March);<br />

V, post-anthesis, ‘fruiting’ (25 April); VI, senescing (2 June); VII, senescent<br />

(6 July); VIII, senescent (28 July); IX, senescent (29 August); X, root<br />

re-growth (4 October); XI, shoot re-growth (4 November); XII, underground<br />

growth (10 December).<br />

The natural rate of narcissus bulb multiplication is low: the average rate of<br />

increase is 1.6 per annum, or 1 to 1000 in 16 years (Rees, 1969). Faster methods of<br />

propagation are available, such as twin-scaling (Hanks <strong>and</strong> Rees, 1979; Fenlon

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