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Narcissus and Daffodil

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Table 11.1 Continued<br />

References<br />

Detector Detection<br />

limit<br />

Mobile phase<br />

(modifier)<br />

Method Test Compoundsa (Internal st<strong>and</strong>ard) Stationary phase or<br />

column<br />

Eckers<br />

et al., 1976<br />

5 ng/ml Claessens<br />

et al., 1983<br />

Ion current<br />

(MS)<br />

UV<br />

(235 nm)<br />

10 ng/ml Claessens<br />

et al., 1988<br />

0.05 µg/ml Tencheva<br />

et al., 1987a<br />

UV<br />

(235 nm)<br />

UV<br />

(280 nm)<br />

1 ng/200 µl Bickel et al.,<br />

1991<br />

Fluorescence<br />

(excitation<br />

290/emission<br />

320 nm)<br />

LC/MS Lycorine, Ambelline, Criglaucine ODS 5 µ Spheresorb Water, Acetonitrile,<br />

Ammonia<br />

HPLC Galanthamine (Phenacetin) CP tm Micro Spher Si n-Hexane-Dichloro-<br />

5 µm<br />

methane-Ethanolamine<br />

HPLC Galanthamine Novapack C-18 (4 µ) Acetonitrile-Pentanesulfonic<br />

acid buffer<br />

HPLC Galanthamine, Epigalanthamine, Hibar-LiChrosorb Methanol-Water<br />

Galanthaminone (Codeine)<br />

RP-8 (5 µm) (Dibutylamine, 85%<br />

Phosphoric acid)<br />

HPLC Galanthamine, Epiganthamine<br />

Intersil-C-8 silica Acetonitrile, Tetra-<br />

(Codeine)<br />

(5 µm)<br />

hydrofuran, Water<br />

(di-n-Butylamine,<br />

85% Phosphoric acid)<br />

Note<br />

a<br />

Reference compounds or those detected.

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