Narcissus and Daffodil

234 C. Codina
mg/g DW
mg/g DW
0.90
0.75
0.60
0.45
0.30
0.15
0.00
0.60
0.50
0.40
0.30
0.20
0.10
0.00
N-formylnorgalanthamine
30 60 90 120 150 180
Sucrose (g/l)
Haemanthamine
30 60 90 120 150 180
Sucrose (g/l)
ts
cm
ts
cm
mg/g DW
mg/g DW
Galanthamine
growth index (at 90 g/l of sucrose) does not coincide with the maximum concentration
of alkaloids (at 30 g/l of sucrose). Although both strains showed similar
growth, the seed-derived shoot-clumps constituted a better kind of plant material
for alkaloid production than those obtained from adult bulbs.
0.90
0.75
0.60
0.45
0.30
0.15
0.00
0.70
0.60
0.50
0.40
0.30
0.20
0.10
0.00
30 60 90 120 150 180
Sucrose (g/l)
Tazettine
30 60 90 120 150 180
Sucrose (g/l)
Figure 7.14 Alkaloid production in seed-derived shoot-clumps grown under different
sucrose concentrations. Bars represent the SD calculated from three replicates.
ts, alkaloids detected in the tissue; cm, alkaloids released to the culture medium.
Table 7.6 Effect of sucrose on alkaloid production (accumulation in both tissue and liquid
medium) in shoot-clumps obtained from bulbs and seeds (values are % DW) a
Sucrose (g/l) Bulb-derived shoot-clumps Seed-derived shoot-clumps
Note
a for abbreviations, see Table 7.1.
NFNGAL GAL HAEM TAZ NFNGAL GAL HAEM TAZ
30 0.028 0.028 0.082 0.015 0.065 0.061 0.057 0.037
60 0.018 0.021 0.041 0.009 0.048 0.040 0.031 0.021
90 0.008 0.021 0.022 0.005 0.045 0.030 0.041 0.043
120 0.014 0.021 0.043 0.008 0.020 0.018 0.025 0.025
150 0.009 0.022 0.032 0.004 0.021 0.023 0.023 0.012
180 0.024 0.047 0.061 0.009 0.012 0.010 0.011 0.008
ts
cm
ts
cm