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Preprint volume - SIBM

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Pre-print Volume - Posters<br />

Topic 2: MARINE ORGANISMS AND ECOSYSTEMS AS MODEL SYSTEMS<br />

Fig. 1 - Survival at endtest and after 48h of S.<br />

aurata embryos exposed to Cd.<br />

Sopravvivenza fine test e 48h post fine<br />

test degli embrioni di S. aurata esposti al<br />

Cd.<br />

Assessing survival 48h post-exposure period, pre-hatched embryos (24 h max aged)<br />

showed LOEL at 0.06 ppm and an EC50 to concentrations more than 0.5 ppm (Fig. 1).<br />

Post-hatched embryos (aged between 72h and 120h) showed higher sensitivity to the<br />

reference toxicant; LOEL value was at 0.007 ppm, with an EC50 less than 0.05 ppm<br />

(Fig. 2).<br />

Conclusions – The lower sensitivity of pre-hatched embryos is probably due to the<br />

presence of chorion. In relation to the large availability of Sparus aurata embryos, this<br />

model can be proposed for a general tool in aquatic biosciences; post-hatched embryos<br />

will be interesting model in ecotoxicological researches and related applications.<br />

References<br />

EMBRY M.R., BELANGER S.E., BRAUNBECK T.A., GALAY-BURGOS M., HALDER M.,<br />

HINTON D.E., LÉONARD M.A., LILLICRAP A., NORBERG-KING T., WHALE G. (2010) -<br />

The fish embryo toxicity test as an animal alternative method in hazard and risk assessment and<br />

scientific research. Aquat. Toxicol., 97 (2): 79-87.<br />

FRANCH R., LOURO B., TSALAVOUTA M, CHATZIPLIS D., TSIGENOPOULOS C.S.,<br />

SARROPOULOU E., ANTONELLO J., MAGOULAS A., MYLONAS C.C., BABBUCCI M.,<br />

PATARNELLO T., POWER D.M., KOTOULAS G., BARGELLONI L. (2006) - A Genetic<br />

Linkage Map of the Hermaphrodite Teleost Fish Sparus aurata L. Genetics, 174: 851-861.<br />

RAFAEL M.S., LAIZÉ V., LEONOR CANCELA M. (2006). Identification of Sparus aurata bone<br />

morphogenetic protein 2: Molecular cloning, gene expression and in silico analysis of protein<br />

conserved features in vertebrates. Bone, 39: 1373–1381.<br />

41 st S.I.B.M. CONGRESS Rapallo (GE), 7-11 June 2010<br />

131<br />

Fig. 2 - Survival at endtest and after 48h of S.<br />

aurata eleutherioembryos exposed to Cd.<br />

Sopravvivenza fine test e 48h post fine test<br />

degli eleuterioembrioni di S. aurata esposti<br />

al Cd.

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