Preprint volume - SIBM

Pre-print Volume - Posters
VARIOUS TOPICS
preabsorption of the I Ab with cyprids crude extract and omission of I Ab. No
immunostaining was detected in controls.
Fig. 1 - IR muscle cells in the thoracic segment. Fig. 2 - IR terminals: muscles and epidermis.
Immunoreattività nel torace. Immunoreattività in muscoli ed
epidermide.
Results - In thoracic muscles (Fig. 1), NMDA R1 immunoreactive (IR) nerve terminals
were localized on muscle cells and ramified in proximity of the nuclei. In the
compound and naupliar eyes ommatidia, the cytoplasmic granules appeared also IR
(not show). It was not determine whether these granules belonged to retinular cells
and/or to pigment cells. The rhabdom of the retinular cells were not IR. Weak IR fibers
were localized within the antennules and finally NMDA R1 immunoreactivity was also
observed in the cytoplasm of epidermal cells (Fig. 2 ).
Conclusions – Considering our results as well as the literature, the B. amphitrite cyprid
neuromuscular junctions should posses NMDA R1 receptors together to GABAergic
receptors, as suggested for crustacean muscular innervations (Feinsten, 2001). Nerve
fibers involved possibly originate from GAD65/67 IR neurons localized in the
posterior ganglion (Gallus et al., 2010). Occurrence of NMDA R1 IR sites in the eyes
and in the epidermal cells agree with the hypothesis that GABA molecules play a role
in non visual eye functions, since the photoreception neurotransmitter appears to be
histamine. Furthermore GABA may be involved in non neural functions of tegument
cells.
References
FEINSTEIN N., PARNAS D., PARNAS H., DUDEL J., PARNAS I. (1998) - Functional and
immunocytochemical identification of glutamate autoreceptors of an NMDA type in crayfish
neuromuscular junction. J. Neurophysiol., 80: 2893-2899.
FEINSTEIN N. (2001) - Immunocytochemical localization of GABAA receptor in Crayfish
neuromuscular junction. The 35 th annual meeting of the Israel society for microscopy. Technion,
Israel Institute of Technology Haifa. May 15, 2001.
http://materials.technion.ac.il/ism/Docs/2001/Feinstein.pdf
GALLUS L., FERRANDO S., GAMBARDELLA C., DIASPRO A., BIANCHINI P., PIAZZA V.,
BONANNO G., MILANESE M., RAMOINO P., TAGLIAFIERRO G. (2010) - The
GABAergic–like system in the Cyprid of Balanus amphitrite (=Amphibalanus amphitrite).
Biofouling, 26: 155-165.
41 st S.I.B.M. CONGRESS Rapallo (GE), 7-11 June 2010
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