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Preprint volume - SIBM

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Pre-print Volume –Posters<br />

NECTON AND FISHERY COMMITEE<br />

TRIGLUC; LEPTCAV-TRIGLUC; TRIPLAS-TRIGLUC; LEPTCAV-TRIGLYR;<br />

LEPTDIE-TRIGLYR.; for “circularity” LEPTDIE-LEPTCAV; LEPTDIE-TRIPLAS;<br />

LEPTCAV-TRIPLAS. Otoliths morphologically more different, defined by three shape<br />

indices, were those belonging to L. cavillone and C. lucerna. The results were in<br />

agreement with the analytical key about the family (FAO, 1987) that placed these two<br />

species at the extremes. 2) MULTIVARIATE ANALYSIS (MANOVA): Intraspecific<br />

comparison were analyzed by multivariate analysis. The otolith outline shape indices<br />

changed with size (between juveniles and adult) and sex for some species. In particular,<br />

intraspecific comparison showed significant differences between juveniles and adults<br />

for A. cuculus, C. lucerna, L. dieuzeidei and between females and males for L.<br />

dieuzeidei. Therefore, about the intraspecific relationship, L. dieuzeidei was the species<br />

more diversified. 3) SCANNING ELECTRON MICROSCOPY (SEM): otolith shape<br />

of triglids were approximately oval. Preliminaries analyses showed interspecific<br />

variations in the morphology and ultrastructure of the sulcus acusticus, about shape,<br />

size, direction of crystalline arrangement and crystalline surface. Intraspecific variation<br />

appeared relate to growth and environmental factors. These results were in agreement<br />

to Tuset et al. (2003) and Jitpukdee (2009) for other families.<br />

Conclusions - These results suggested: similarity between species belonged to the<br />

same genus; differences between species phylogenetically distant (e.g. L. cavillone and<br />

C. lucerna); intraspecific differences between juveniles and adults (e.g. A. cuculus, C.<br />

lucerna, Lepidotrigla dieuzeidei); similarity between species belonged to different<br />

genera (Lepidotrigla spp. and C. lastoviza). These methods (geometric morphometrics<br />

and ultrastructure analysis) constitute an important instrument for species identification<br />

using sagittal otoliths collected in feeding remains of bony fish predators, in<br />

fossiliferous layers, in archaeological sites.<br />

References<br />

AMORIM M.C.P., STRATOUDAKIS Y., HAWKINS A.D. (2004) - Sound production during<br />

competitive feeding in the grey gurnard. J. Fish Biol., 65: 182-194.<br />

JITPUKDEE S. (2009) - Crystalline structure of sagittal otoliths from 8 fish species in different<br />

habitats. J. Micro. Soc. Thai., 23 (1): 57-61.<br />

R DEVELOPMENT CORE TEAM (2010) - R: A language and environment for statistical<br />

computing. R Foundation for Statistical Computing, Vienna, Austria. http://www.R-project.org.<br />

RASBAND W.S. (2008) - IMAGEJ.U.S. National Institutes of Health, Bethesda, Maryland, USA.<br />

http://rsb.info.nih.gov/ij/.<br />

TUSET V.M., LOMBARTE A., GONZALEZ J.A., PERTUSA J.F., LORENTES M.J. (2003) -<br />

Comparative morphology of the sagittal otolith in Serranus spp. J. Fish. Biol., 63: 1491-1504.<br />

VALLISNERI M., MONTANINI S., STAGIONI M. (2010) - Length-weight relationships for the<br />

family Triglidae in the Adriatic Sea, northeastern Mediterranean. J. Appl. Ichthyol., 26 (1-3).<br />

41 st S.I.B.M. CONGRESS Rapallo (GE), 7-11 June 2010<br />

327

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