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Preprint volume - SIBM

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Pre-print Volume – Posters<br />

AQUACULTURE COMMITEE<br />

Results and conclusions – Starvation caused a general, although not significant,<br />

decrease of digestive enzymes, with an earlier recovery (on T2) to initial values for<br />

total proteases in the midgut and delayed (on T3) for amylase and lipase (Tab. 1).<br />

Reversibility was also observed in Atlantic salmon (Krogdahl & Bakke-McKellep,<br />

2005) and in sturgeon and in trout (Furné et al., 2008). No significant changes in<br />

haematological and biochemical parameters were observed. This suggested the good<br />

resistance of red porgy to short-term starvation.<br />

Tab. 1 - Specific digestive enzyme activities (U/mg protein) of Pagrus pagrus.<br />

Attività specifica (U/mg proteina) degli enzimi digestivi di Pagrus pagrus.<br />

Starved - refed group Fed group<br />

Times Stomach Proximal Midgut Distal gut Stomach Proximal Midgut Distal gut<br />

gut<br />

gut<br />

Total proteases<br />

T0 0,583 0,739 0,336 0,778<br />

T1 0,468 0,320 0,440 0,465 0,879 1,079 1,041 0,724<br />

T2 0,617 0,266 0,668 0,430 1,936 1,155 1,145 1,997<br />

T3<br />

Amylase<br />

1,045 0,802 0,911 0,511 2,004 1,676 2,269 2,601<br />

T0 0,129 0,205 0,026 0,061<br />

T1 0,021 0,063 0,034 0,007 0,124 0,104 0,040 0,025<br />

T2 0,017 0,104 0,029 0,018 0,113 0,201 0,044 0,109<br />

T3<br />

Lipase<br />

0,031 0,186 0,026 0,065 0,230 0,297 0,049 0,147<br />

T0 0,006 0,0008 0,0009<br />

T1 0,001 0,0002 0,0007 0,001 0,0007 0,0014<br />

T2 0,001 0,0005 0,0011 0,003 0,0014 0,0071<br />

T3 0,004 0,0007 0,0036 0,010 0,0101 0,0070<br />

References<br />

CARUSO G., DENARO M.G., GENOVESE L. (2009) - Digestive enzymes in some Teleost species<br />

of interest for Mediterranean aquaculture. The Open Fish Sci. J., 2: 74-86.<br />

CARUSO G., MARICCHIOLO G., MICALE V., GENOVESE L., CARUSO R., DENARO M.G.<br />

(2008) - Physiological responses to starvation in European eel (Anguilla anguilla): effects on<br />

haematological, biochemical, non-specific immune parameters and skin structure. Fish Physiol.<br />

Biochem. DOI 10.1007/S10695-008-9290-6.<br />

FURNÈ M., GARCIA-GALLEGO M., HIDALGO M.C., MORALES A.E., DOMEZAIN A.,<br />

DOMEZAIN J., SANZ A. (2008) - Effect of starvation and refeeding on digestive enzymes<br />

activities in sturgeon (Acipenser naccarii) and trout (Oncorhynchus mykiss). Comp. Biochem.<br />

Physiol. Part A, 149: 420-425.<br />

KROGDHAL A., BAKKE-MCKELLEP A.M. (2005) - Fasting and re-feeding cause rapid changes in<br />

intestinal tissue mass and digestive enzyme capacities of Atlantic salmon (Salmo salar L.).<br />

Comp. Biochem. Physiol. Part A, 141: 450-460.<br />

41 st S.I.B.M. CONGRESS Rapallo (GE), 7-11 June 2010<br />

242

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