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NIS - libdoc.who.int - World Health Organization

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Herba Equiseti<br />

nate) scales, forming a specific vaginate structure in the nodes of the<br />

branches, which is usually called a “sheath” (1, 2, 28, 29).<br />

Organoleptic properties<br />

Odour: slight or no odour; taste: slightly acidic or no taste (2, 28, 29).<br />

Microscopic characteristics<br />

Epidermis with elongated, wavy-walled cells, silicified, often thickened<br />

and beaded. The paracytic stomata with the 2 subsidiary cells cover the<br />

guard cells and have conspicuous radial ridges. In transverse sectional<br />

view the epidermis is crenate, with the protuberances formed from the<br />

contiguous mass of 2 U-shaped cells. (No single-celled protuberances<br />

should be present – absence of E. palustre L.) The cortex of stems of<br />

thin-walled cells with many large lacunae; non-lignified, collenchymatous<br />

fibrous cells up to 1 mm long with oblique or bifurcate apices in the<br />

ridges; xylem of lignified, annularly or spirally thickened vessels occurring<br />

singly or in small groups; slightly lignified annularly thickened tracheids<br />

and bundles of long non-lignified fibres with narrow lumens.<br />

Large parenchymatous cells packed with minute starch grains; large<br />

central cavity. Branches have four vascular bundles and lack a central<br />

cavity; the silicified collenchyma is reduced, cortex lacunae are absent<br />

(1, 28–31).<br />

Powdered plant material<br />

Greenish-grey or dull green. The powder shows characteristic elements<br />

of stems (see Microscopic characteristics) (1, 28, 29).<br />

General identity tests<br />

Macroscopic and microscopic examinations, and thin-layer chromatography<br />

for the characteristic constituents: caffeic acid, hyperoside and rutin<br />

(1, 28), quercetin 3-glucoside (5) and flavone-5-glucosides (2).<br />

Purity tests<br />

Microbiological<br />

Tests for specific microorganisms and microbial contamination limits are<br />

as described in the WHO guidelines on quality control methods for medicinal<br />

plants (32).<br />

Chemical<br />

No information available.<br />

115

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