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NIS - libdoc.who.int - World Health Organization

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Flos Helichrysi arenarii<br />

General identity tests<br />

Macroscopic and microscopic examinations, chemical analysis and thinlayer<br />

chromatography tests for flavonoids, which are the characteristic<br />

constituents (1, 2, 5).<br />

Purity tests<br />

Microbiological<br />

Tests for specific microorganisms and microbial contamination limits are<br />

as described in the WHO guidelines on quality control methods for medicinal<br />

plant materials (19).<br />

Chemical<br />

The thin-layer chromatogram must not show an orange zone above the<br />

brownish main zone (purity test for other Helichrysum species) (2).<br />

Foreign organic matter<br />

Foreign organic matter not more than 0.5%. Maximum 5% of flower<br />

heads with long flower stalks greater than 1 cm. Not more than 5% of<br />

broken flower heads, with a diameter less than 2 mm (1). Absence of Helichrysum<br />

angustifolium DC and H. stoechas flower heads as adulterants<br />

(5). Foreign matter, not more than 2% (2, 20); absence of H. italicum<br />

(Roth) Guss. and H. stoechas flower heads (20).<br />

Total ash<br />

Not more than 8% (1). Not more than 7% (2).<br />

Acid-insoluble ash<br />

No information available.<br />

Sulfated ash<br />

Not more than 8.5% (20).<br />

Water-soluble extractive<br />

No information available.<br />

Alcohol-soluble extractive<br />

No information available.<br />

Loss on drying<br />

Not more than 12% (1). Not more than 10% (21).<br />

177

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