NIS - libdoc.who.int - World Health Organization

Fructus Hippophaës recens
mis has corymb-like trichomes characteristic for the Elaeagnaceae. The
corymb-like trichomes have a continuous multicellular disc with ray-serrate
edge and a multicellular stalk. The trichome stalk consists of 6–8 radial
cells, which surround one or a few (2–4) smaller cells; there are numerous
solitary stalks after detaching of the broken corymb-like disc. Multicellular
stalks are visible through the transparent corymbs. The mesocarp of ripened
fruit is liquefied. It represents a mixture of entire cells, cytoplasm, oil
droplets, chromoplasts and chaotic vascular bundles. The calyx of the fruit
is slightly open. There are hairs in the calyx opening, in the seed cavity and
on the tail of the seed sac. The peduncle has an epidermis with corymb-like
trichomes and external thick-walled cells, cortical parenchyma with sclerenchymatic
cells and some primary vascular bundles arranged in circles.
The achene, the true fruit, usually known as the “seed”, consists of
tegument, perisperm and endosperm. The tegument is formed by thickwalled
palisade cells, perpendicularly arranged on 2–3 layers of compressed
parenchyma. The perisperm consists of 3–4 layers of compressed
small thin-walled cells. Endosperm is a range of cells containing aleurone.
The palisade cells of the cotyledons contain oil and aleurone (2, 37, 38).
Powdered plant material
Not applicable to fresh berries.
General identity tests
Macroscopic and microscopic examinations, chemical analysis and thinlayer
chromatography tests for the characteristic constituents, isorhamnetin
and quercetin (39). Flavonoids may be rapidly determined by capillary
zone electrophoresis (40).
Purity tests
Microbiological
Tests for specific microorganisms and microbial contamination limits are
as described in the WHO guidelines on quality control methods for medicinal
plant materials (41).
Chemical
A chromatographic analytical method has been described (42).
Foreign organic matter
Not more than 1% fragments of stems and other parts of plant. Not more
than 1% of unripe berries. Not more than 2% of fruits damaged by ver-
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