NIS - libdoc.who.int - World Health Organization

WHO monographs on medicinal plants commonly used in the Newly Independent States (NIS)
yellow zone of sanguinarine. The pale white fluorescent zone of protopine
is located in the same position as standard berberine: this is usually
overlapped by sanguinarine after treatment with Dragendorff-sodium nitrite;
chelidonine (Rf approximately 0.6) and the other alkaloids from
rapidly fading brown zones. Papaverine can be used for the comparison
of Rf values (42). The addition of 1 ml of Dragendorff’s reagent to the
dried residue of a chloroform extract results in a golden precipitate. Addition
of one drop of chloroform extract to the filter paper shows an area
of fluorescence under an ultraviolet lamp (43).
Purity tests
Microbiological
Tests for specific microorganisms and microbial contamination limits are
as described in the WHO guidelines on quality control methods for medicinal
plants (44).
Chemical
No information available.
Foreign organic matter
Not more than 1% of organic matter. Not more than 3% of aerial parts
with non-natural colour (2).
Total ash
Not more than 13% (1). Not more than 15% (2, 5).
Acid-insoluble ash
Not more than 2% (2, 5).
Sulfated ash
No information available.
Water-soluble extractive
Not less than 20% (5).
Alcohol-soluble extractive
No information available.
Loss on drying
Not more than 10% (1). Not more than 14% (2).
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