Clinical Biochemistry of Domestic Animals (Sixth Edition) - UMK ...

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Chapter | 6 Clinical Veterinary Immunology
FIGURE 6-5 Plate agglutination test for rheumatoid factor, using a test kit that couples IgG to latex beads.
Serum is mixed with beads and examined for agglutination and compared with controls.
likely show up as positive on the ELISA because of the
increased sensitivity of the latter assay. This type of double
immunodiffusion (Ouchterlony) is also used in the demonstration
of precipitating antibodies against some fungal
pathogens, such as Aspergillus fumigatus and Coccidioides
immitis . In both cases, positive control sera are used to
demonstrate identity with potentially positive sera.
E . Indirect and Direct Immunofluorescence
(IFA) Test
The indirect immunofluorescence assay (IFA) is a technique
that is used to diagnose some protozoan and viral
diseases. It is also the basis of the antinuclear antibody test,
used to diagnosis autoimmune disease. It is useful when