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Clinical Biochemistry of Domestic Animals (Sixth Edition) - UMK ...

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Appendix IV<br />

Stability <strong>of</strong> Serum Enzymes under<br />

Various Storage Conditions a<br />

Enzyme 25EC 4EC 25EC<br />

Acid phosphatase 4 h b 3 d c 3 d c<br />

Alkaline phosphatase 2–3 d<br />

d<br />

2–3 d 1 month<br />

Aldolase 2 d 2 d Unstable e<br />

Amylase 1 month 7 month s 2 months<br />

Cholinesterase 1 wk 1 wk 1 wk<br />

Creatine kinase 2 d 1 wk 1 month<br />

γ -Glutamyl transferase 2 d 1 wk 1 month<br />

Glutamate dehydrogenase 1 d 2 d 1 d<br />

Aspartate aminotransferase 3 d 1 wk 1 month<br />

Alanine aminotransferase 2 d 1 wk Unstable<br />

e<br />

Hydroxybutyrate dehydrogenase Unstable 3 d Unstable<br />

e<br />

Isocitrate dehydrogenase 5 h 3 d 3 wk<br />

Leucine aminopeptidase 1 wk 1 wk 1 wk<br />

Lactate dehydrogenase 1 wk 1–3 d f 1–3 d f<br />

Malate dehydrogenase Unstable 3 d 3 d<br />

Sorbitol (Iditol)dehydrogenase Unstable 1 d 2 d<br />

a<br />

No more than 10% <strong>of</strong> the original activity lost during the specifi ed time.<br />

b<br />

At pH 5–6.<br />

c<br />

With added citrate or acetate.<br />

d<br />

Activity may increase.<br />

e<br />

Enzyme does not tolerate thawing well.<br />

f<br />

Depends on the isoenzyme pr<strong>of</strong>i le.<br />

878

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