Clinical Biochemistry of Domestic Animals (Sixth Edition) - UMK ...

Appendix VI
Nomogram for Computing Relative
Centrifugal Forces (RCF)
25,000
20,000
15,000
10,000
6,000
4,000
3,000
2,000
1,000
500
200
Speed of
centrifuge
spindle
in R.P.M
30,000
20,000
10,000
5,000
2,000
1,000
500
200
100
50
20
10
3
RCF (xg)
Example line
50
45
40
35
30
25
20
15
15
14
13
12
11
10
9
8
7
6
5
Radius in cm
from center
of centrifuge
spindle
FIGURE VI-1 To calculate the RCF value at any point along the tube,
measure the radius in cm from the center of the centrifuge spindle to
the point. Draw a line from this radius value on the right-hand column to
the centrifuge speed on the left-hand column. The RCF value is the point
where the line intersects the center column. The nomogram is based on
the following formula:
RCF 0.00001118 radius RPM 2 .
880