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Part-IResults and DiscussionDrug Preparation & Treatment:Omeprazole (30 mg/kg) & test compounds were suspended in 1% suspension of acacia indistilled water and administered by oral route.The animals were fasted for 24 hrs prior the experiment, but had free access to water.After the fasting period, the animals were given the drug samples p.o, 1 hr. prior theligation. Thereafter, the rats were anaesthetized with anesthetic ether. After an hour, eachof the rats was secured on the operating table. An incision of 1 cm length in the abdomenjust below the sternum was made. The stomach was exposed. A thread was passed aroundthe pyloric sphincter and a light knot was applied to it taking due care that no bloodvessel was tied along with the knot. After this, the incision was closed by stitching theabdominal wall by a thread. The underlying skin was cleaned of any bleeding etc. Anantiseptic cream was applied over the wound. Thereafter, the animal was kept in aseparate cage and allowed to recover.After 24 hrs, these animals were sacrificed and the stomach of each of the animals wasisolated and cut open through its greater curvature. The gastric contents were carefullyremoved.Following parameters were studied1. Volume of gastric juice secreted: The volume of gastric juice was measured andcentrifuged at 1000 rpm for 10 min.2. Determination of total acidity of the gastric juice: From the supernatant, aliquots(1 ml of each) were taken for the determination of total acidity.3. The ulcer index: gastric mucosa was also examined for ulcers.4. The pH of the gastric secretion was measured by digital pH meter (Equipt-TronicsDigital pH meter, Model EQ-610).Determination of total acidity: An aliquot of 1 ml of gastric juice was taken in a 50 mlconical flask then dilute with distill water to make volume 10 ml and 2 drops ofphenolphthalein indicator was added to it. It was further titrated with 0.01 N NaOH untila permanent pale pink color was developed.The volume of alkali consumed was noted. The total acidity is expressed as mEq./lt/gmby the following formula:Total Acidity = Vol. of NaOH consumed x N x 100 / 0.1 (mEq/100gm)119