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View/Open - ARAN - National University of Ireland, Galway

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Chapter 3<br />

3.4.3. Intra-serovar variation in bi<strong>of</strong>ilm recovered after 168 hours<br />

Table 3.3 displays the mean log 10 density <strong>of</strong> bi<strong>of</strong>ilm recovered from the 5<br />

surfaces after 168 hour bi<strong>of</strong>ilm formation. As displayed, more cells were<br />

recovered from the S. Agona outbreak strain S09-0494 bi<strong>of</strong>ilm than the<br />

bi<strong>of</strong>ilm formed by the S. Agona reference strain SL483 on all 5 surfaces<br />

(p=0.004 on all 5 surfaces). There was over a 1 log 10 CFU/coupon difference<br />

in the number <strong>of</strong> cells recovered from the S. Agona S09-0494 in<br />

comparison to the S. Agona SL483 strain when the bi<strong>of</strong>ilm was formed on<br />

glass, steel, polycarbonate when the bi<strong>of</strong>ilm was formed over 168 hours.<br />

The differences between S. Typhimurium S09-0419 and S. Typhimurium<br />

reference strain SL1344 were less consistent as more cells were only<br />

recovered from the S. Typhimurium S09-0419 bi<strong>of</strong>ilm on glass and tile.<br />

Page<br />

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