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Appendix 4 Poster Presentation Environmental Research Institute – June 2012. Title: Prevention of Food-borne Salmonellosis: The challenge of killing a biofilm. Authors: M. Corcoran, D. Morris, N. De Lappe, J. O’Connor, P. Lalor, P. Dockery and M. Cormican. Location: NUI, Galway. Oral Presentation at School of Medicine Annual Research Day – May 2012. Title: Biofilm age can impact the efficacy of disinfectants. Location: NUI, Galway. Authors: M. Corcoran, D. Morris, N. De Lappe, J. O’Connor, P. Lalor, P. Dockery and M. Cormican. Awarded second prize for best presentation of research. Poster Presentation at School of Medicine Annual Research Day – May 2012. Title: Killing an established biofilm. Location: NUI, Galway. Authors: M. Corcoran, D. Morris, N. De Lappe, J. O’Connor, P. Lalor, P. Dockery and M. Cormican. Poster Presentation at Federation of Infections Society— November 2011. Title: Salmonella enterica biofilm: older biofilms are different. Authors: M. Corcoran, D. Morris, N. De Lappe, J. O’Connor, P. Lalor, P. Dockery and M. Cormican. Location: Manchester, UK. Oral Presentation at British Society for Antimicrobial Chemotherapy – November 2011. Antimicrobial Resistance Mechanisms Workshop. Title: How to kill a Salmonella Biofilm. Authors: M. Corcoran, D. Morris, N. De Lappe, J. O’Connor, P. Lalor, P. Dockery and M. Cormican. Location: Birmingham, UK. Received full sponsorship through BSAC Student sponsorship programme to travel and present my work at the workshop. Poster Presentation at American Society for Microbiology – May 2011. Title: Variation in Salmonella enterica Serovars Ability to Establish a Biofilm. Location: New Orleans, Louisiana, USA. Received a travel grant worth $500, based on the ASM committee review of abstracts. Oral Presentation at Society of General Microbiology – April 2011. Title: Salmonella enterica serovar Enteritidis biofilm formation on 5 surfaces found in food processing environments. Authors: M. Corcoran, D. Morris, N. De Lappe, J. O’Connor, P. Lalor, P. Dockery and M. Cormican. Location: Queens University Belfast, UK. Page 267
Appendix 4 Poster Presentation at Society for General Microbiology – April 2011. Title: Differences in the biofilm properties of laboratory adapted and recent isolates of Salmonella enterica. Authors: M. Corcoran, D. Morris, N. De Lappe, J. O’Connor, P. Lalor, P. Dockery and M. Cormican. Location: International Conference Centre, Harrogate, UK. Oral Presentation at Society for General Microbiology — April 2011. Title: The ability of Salmonella enterica to form a biofilm on food contact surfaces. Authors: M. Corcoran, D. Morris, N. De Lappe, J. O’Connor, P. Lalor, P. Dockery and M. Cormican. Location: University of Nottingham, UK. Received a travel grant worth £320 pounds for attending the conference. Oral Presentation at International Association of Food Protection – June 2010. Title: Differences in the ability of contact materials to support Salmonella enterica biofilm formation. Authors: M. Corcoran, D. Morris, N. De Lappe, J. O’Connor, P. Lalor, P. Dockery and M. Cormican. Location: University College Dublin, Ireland. Poster Presentation at Society for General Microbiology – April 2010. Title: Establishing a Laboratory model of Salmonella enterica biofilm. Authors: M. Corcoran, D. Morris, N. De Lappe, J. O’Connor, P. Lalor, P. Dockery and M. Cormican. Location: NUI, Galway. Page 268
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Salmonella enterica - biofilm forma
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Table of Contents Page Number 1.16.
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Table of Contents Page Number 4.1.2
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Table of Contents Page Number 6.8.4
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List of Abbreviations Table Page Nu
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List of Abbreviations List of Abbre
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Summary of Content Summary of Conte
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“To show your true ability is alw
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Acknowledgements ever met, Fiona th
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Acknowledgements This Ph.D. thesis
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Chapter 1 1.1. Salmonella Salmonell
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Chapter 1 may impose enormous costs
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Chapter 1 cracks or penetration of
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Chapter 1 detected by agglutination
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Chapter 1 become colonized with >10
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Chapter 1 antigens [z27],[z45] [1].
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Chapter 1 S. Agona has also been im
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Chapter 1 isolated from undercooked
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Chapter 1 The S. Typhimurium strain
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Chapter 1 ecosystem with the use of
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Chapter 1 [82]. Previous research h
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Chapter 1 to genes involved in flag
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Chapter 1 observation has also been
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Chapter 1 [119] and flow cell react
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Chapter 1 based disinfectant) the s
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Chapter 1 formation of two strains
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Chapter 1 shield the S. Agona cells
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Chapter 1 Key Objectives of this st
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Chapter 2 2. Abstract Food-borne pa
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Chapter 2 indicated that there may
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Chapter 2 taken from industrial set
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Chapter 2 2.1.4. CDC Biofilm Reacto
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Chapter 2 can be performed without
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Chapter 2 To summarise, previous au
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Chapter 2 2.3. Methods for examinin
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Chapter 2 and the biofilm formed th
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Chapter 2 2.4. Statistical Analysis
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Chapter 2 Table 2.1: Strain charact
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Chapter 2 XII. The gas port and med
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Chapter 2 II. Primary fixative cons
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Chapter 2 IX. Once the conditions w
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Chapter 2 Figure 2.1: Image of CBR
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Chapter 2 2.6. Results 2.6.1. Asses
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Chapter 2 2.6.3. SEM Analysis of su
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Chapter 2 Figure 2.6: Complete remo
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Chapter 2 2.6.5. Mean log 10 densit
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Chapter 2 2.6.6. Mean log 10 densit
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Chapter 2 Table 2.5: Mean log 10 de
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Chapter 2 The results displayed in
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Chapter 2 Table 2.7: Difference bet
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Chapter 2 Table 2.8: The mean log 1
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Chapter 2 Figure 2.7: Graph of the
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Chapter 2 also used “high” soni
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Chapter 2 2.8. Summary All 13 strai
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Chapter 3 3. Abstract It has been e
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Chapter 3 classified as persistent
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Chapter 3 was plated onto TSA for e
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Chapter 3 Figure 3.1: The mean log
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Chapter 3 Figure 3.3: SEM image of
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Chapter 3 Table 3.2: Difference bet
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Chapter 3 3.4.3. Intra-serovar vari
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Chapter 3 3.4.4. Strain variation i
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Chapter 3 3.4.5. The density of bio
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Chapter 3 3.5. Discussion As illust
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Chapter 3 As discussed in section 3
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Chapter 3 increased biofilm appeare
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Chapter 4 Examining the efficacy of
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Chapter 4 through laboratory based
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Chapter 4 acid and quaternary ammon
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Chapter 4 the surface the temperatu
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Chapter 4 availability of multiple
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Chapter 4 peracetic acid (100, 200,
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Chapter 4 As discussed previously,
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Chapter 4 Dey/Engley (Difco) neutra
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Chapter 4 sterilisation was achieve
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Chapter 4 IX. Aliquots of 100µl of
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Chapter 4 XV. XVI. XVII. XVIII. XIX
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Chapter 4 4.3. Results 4.3.1. Suspe
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Chapter 4 Table 4.3: Mean log 10 de
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Chapter 4 Benzalkonium chloride was
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Chapter 4 4.4. Discussion Suspensio
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Chapter 4 effective at eliminating
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Chapter 4 Surprisingly, Nguyen et a
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Chapter 4 though a contact time of
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Chapter 4 provide a more standardis
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Chapter 4 hours instead of the stan
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Chapter 5 5. Abstract Examining bio
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Chapter 5 dry and rough (bdar) morp
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Chapter 5 absence of curli and cell
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Chapter 5 studies such as the micro
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Chapter 5 discussed in chapter 4. T
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Chapter 5 5.2. Methods 5.2.1. Colon
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Chapter 5 XIII. XIV. XV. XVI. XVII.
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Chapter 5 XVI. XVII. XVIII. XIX. XX
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Chapter 5 5.3.2. Repeatability of m
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Chapter 5 Figure 5.2: Stained biofi
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Chapter 5 was formed at room temper
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Chapter 5 Table 5.3: The density of
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Chapter 5 5.3.4. The density of bio
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Chapter 5 Table 5.6: The difference
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Chapter 5 Table 5.7: The difference
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Chapter 5 Table 5.8: Summary of ass
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Chapter 5 interpretation therefore
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Chapter 5 measures of S. enterica b
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Chapter 5 cells peaks [165, 191]. D
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Chapter 5 5.5. Limitations of the s
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Chapter 6 Discussion of S. enterica
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Chapter 6 However, a number of auth
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Chapter 6 It is of interest to exam
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Chapter 6 were similar at 48 hours.
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Chapter 6 attributable to a small n
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Chapter 6 chosen as an appropriate
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Chapter 6 undetermined. However, th
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Chapter 6 reason for this may be du
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Chapter 6 Moreover, based on the re
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Chapter 6 responsible for increased
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Chapter 6 not fully capture the ran
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Chapter 6 However, the extent of bi
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Page 242 and 243: Bibliography Bibliography Page 221
Page 244 and 245: Bibliography 12. T. Takata, J.L., H
Page 246 and 247: Bibliography 29. Barker, J., M. Nae
Page 248 and 249: Bibliography 47. Threlfall, E.J., H
Page 250 and 251: Bibliography 65. CDC. Investigation
Page 252 and 253: Bibliography 83. Ledeboer, N., Frye
Page 254 and 255: Bibliography 100. Chia, T., Goulter
Page 256 and 257: Bibliography 117. Buckingham-Meyer,
Page 258 and 259: Bibliography 133. European Committe
Page 260 and 261: Bibliography Supernatant of a Hafni
Page 262 and 263: Bibliography 165. Díez-García, M.
Page 264 and 265: Bibliography cultivation of Staphyl
Page 266 and 267: Bibliography Compounds. 2012. Appli
Page 268 and 269: Bibliography 215. Buck, et al., A q
Page 270 and 271: Bibliography Canadian Journal of Ps
Page 272 and 273: Appendix 1 Appendix 1— List of Re
Page 274 and 275: Appendix 1 Appendix 1 - List of Equ
Page 276 and 277: Appendix 2 Colour index High outlie
Page 286 and 287: Appendix 3 Appendix 3-Figure 2: PFG
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